Stripping Voltammetric Determination of Choline Based on Micro-Fabricated Composite Membrane

Hye Jin Lee, Carine Beriet, Hubert H. Girault

Research output: Contribution to journalArticlepeer-review

42 Scopus citations

Abstract

A novel stripping voltammetric method for the determination of non-redox ionic species is presented. Using choline ion (Ch+) as an example, we demonstrate how a micro machined PVC-NPOE gel membrane can be used to accumulate Ch+ ion under potentiostatic steady-state conditions. The amperometric stripping of the target ion can be carried out by any voltammetric method but we presently illustrate the principle using linear sweep voltammetry. The effect of accumulation time, accumulation potential and choline ion concentration has been examined. The present work also includes the quantitative study of an enzyme reaction generating choline, namely the reaction of calcium phosphocholine with alkaline phosphatase. With this experimental approach, (μg ml-1 concentration level of alkaline phosphatase enzyme can be determined.

Original languageEnglish
Pages (from-to)71-77
Number of pages7
JournalAnalytical Sciences
Volume14
Issue number1
DOIs
StatePublished - 1998

Keywords

  • Alkaline phosphatase
  • Choline
  • Excimer laser
  • Ion transfer
  • Polymer membrane
  • Stripping voltammetry

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