TY - JOUR
T1 - Suppression of arachidonic acid metabolism and nitric oxide formation by kudzu isoflavones in murine macrophages
AU - Jun, Mira
AU - Hong, Jungil
AU - Jeong, Woo Sik
AU - Ho, Chi Tang
PY - 2005/12
Y1 - 2005/12
N2 - Inhibitory effect of kudzu isoflavones on arachidonic acid metabolism and nitric oxide (NO) production in lipopolysaccharide activated RAW 264.7 macrophages were investigated. Isoflavone aglycones, such as daidzein, genistein, biochanin A, and formononetin significantly suppressed arachidonic acid release (50 μM). Biochanin A, which displayed the most active inhibition on arachidonic acid release in HT-29 human colon cancer cells, exhibited its most potent suppression in RAW 264.7 cell (by 86%) without showing cytotoxicity. However, isoflavone glucosides, puerarin and daidzin, showed lower inhibitory activities on the release of arachidonic acid and its metabolites. In NO formation, biochanin A showed marked inhibition, by 62% (50 μM), followed by genistein, daidzein, formononetin, and daidzin, 56, 39, 33, and 8%, respectively. 5,7-Dihydroxyl group in the A-ring of isoflavones could be a key functional group responsible for the strong inhibitory activity of biochanin A and genistein on NO production. These activities may contribute to the antiinflammatory and anticarcinogenic properties of kudzu isoflavones.
AB - Inhibitory effect of kudzu isoflavones on arachidonic acid metabolism and nitric oxide (NO) production in lipopolysaccharide activated RAW 264.7 macrophages were investigated. Isoflavone aglycones, such as daidzein, genistein, biochanin A, and formononetin significantly suppressed arachidonic acid release (50 μM). Biochanin A, which displayed the most active inhibition on arachidonic acid release in HT-29 human colon cancer cells, exhibited its most potent suppression in RAW 264.7 cell (by 86%) without showing cytotoxicity. However, isoflavone glucosides, puerarin and daidzin, showed lower inhibitory activities on the release of arachidonic acid and its metabolites. In NO formation, biochanin A showed marked inhibition, by 62% (50 μM), followed by genistein, daidzein, formononetin, and daidzin, 56, 39, 33, and 8%, respectively. 5,7-Dihydroxyl group in the A-ring of isoflavones could be a key functional group responsible for the strong inhibitory activity of biochanin A and genistein on NO production. These activities may contribute to the antiinflammatory and anticarcinogenic properties of kudzu isoflavones.
KW - Arachidonic acid
KW - Isoflavones
KW - Kudzu
KW - Macrophage
KW - Nitric oxide
UR - http://www.scopus.com/inward/record.url?scp=29344443799&partnerID=8YFLogxK
U2 - 10.1002/mnfr.200500103
DO - 10.1002/mnfr.200500103
M3 - Article
C2 - 16254887
AN - SCOPUS:29344443799
SN - 1613-4125
VL - 49
SP - 1154
EP - 1159
JO - Molecular Nutrition and Food Research
JF - Molecular Nutrition and Food Research
IS - 12
ER -
