Suppression of NS3 and MP is important for the stable inheritance of RNAi-mediated rice stripe virus (RSV) resistance obtained by targeting the fully complementary RSV-CP gene

Hyang Mi Park; Man Soo Choi; Do Yeon Kwak; Bong Choon Lee; Jong Hee Lee; Myeong Ki Kim; Yeon Gyu Kim; Dong Bum Shin; Soon Ki Park; Yul Ho Kim

doi:10.1007/s10059-012-2185-5

Suppression of NS3 and MP is important for the stable inheritance of RNAi-mediated rice stripe virus (RSV) resistance obtained by targeting the fully complementary RSV-CP gene

Hyang Mi Park, Man Soo Choi, Do Yeon Kwak, Bong Choon Lee, Jong Hee Lee, Myeong Ki Kim, Yeon Gyu Kim, Dong Bum Shin, Soon Ki Park, Yul Ho Kim

Rural Development Administration

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Rice stripe virus (RSV) is a viral disease that seriously impacts rice production in East Asia, most notably in Korea, China, and Japan. Highly RSV-resistant transgenic japonica rice plants were generated using a dsRNAi construct designed to silence the entire sequence region of the RSV-CP gene. Transgenic rice plants were inoculated with a population of viruliferous insects, small brown planthoppers (SBPH), and their resistance was evaluated using ELISA and an infection rate assay. A correlation between the expression of the RSV-CP homologous small RNAs and the RSV resistance of the transgenic rice lines was discovered. These plants were also analyzed by comparing the expression pattern of invading viral genes, small RNA production and the stable transmission of the RSV resistance trait to the T3 generation. Furthermore, the agronomic trait was stably transmitted to the T4 generation of transgenic plants.

Original language	English
Pages (from-to)	43-51
Number of pages	9
Journal	Molecules and Cells
Volume	33
Issue number	1
DOIs	https://doi.org/10.1007/s10059-012-2185-5
State	Published - Jan 2012

Keywords

Movement protein (MP)
Oryza sativa L.
Rice stripe virus (RSV)
RNAi
RSV coat protein
Silencing suppressor (NS3)
Small brown planthopper (SBPH)

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Park, H. M., Choi, M. S., Kwak, D. Y., Lee, B. C., Lee, J. H., Kim, M. K., Kim, Y. G., Shin, D. B., Park, S. K., & Kim, Y. H. (2012). Suppression of NS3 and MP is important for the stable inheritance of RNAi-mediated rice stripe virus (RSV) resistance obtained by targeting the fully complementary RSV-CP gene. Molecules and Cells, 33(1), 43-51. https://doi.org/10.1007/s10059-012-2185-5

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title = "Suppression of NS3 and MP is important for the stable inheritance of RNAi-mediated rice stripe virus (RSV) resistance obtained by targeting the fully complementary RSV-CP gene",

abstract = "Rice stripe virus (RSV) is a viral disease that seriously impacts rice production in East Asia, most notably in Korea, China, and Japan. Highly RSV-resistant transgenic japonica rice plants were generated using a dsRNAi construct designed to silence the entire sequence region of the RSV-CP gene. Transgenic rice plants were inoculated with a population of viruliferous insects, small brown planthoppers (SBPH), and their resistance was evaluated using ELISA and an infection rate assay. A correlation between the expression of the RSV-CP homologous small RNAs and the RSV resistance of the transgenic rice lines was discovered. These plants were also analyzed by comparing the expression pattern of invading viral genes, small RNA production and the stable transmission of the RSV resistance trait to the T3 generation. Furthermore, the agronomic trait was stably transmitted to the T4 generation of transgenic plants.",

keywords = "Movement protein (MP), Oryza sativa L., Rice stripe virus (RSV), RNAi, RSV coat protein, Silencing suppressor (NS3), Small brown planthopper (SBPH)",

author = "Park, {Hyang Mi} and Choi, {Man Soo} and Kwak, {Do Yeon} and Lee, {Bong Choon} and Lee, {Jong Hee} and Kim, {Myeong Ki} and Kim, {Yeon Gyu} and Shin, {Dong Bum} and Park, {Soon Ki} and Kim, {Yul Ho}",

year = "2012",

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doi = "10.1007/s10059-012-2185-5",

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AU - Choi, Man Soo

AU - Kwak, Do Yeon

AU - Lee, Bong Choon

AU - Lee, Jong Hee

AU - Kim, Myeong Ki

AU - Kim, Yeon Gyu

AU - Shin, Dong Bum

AU - Park, Soon Ki

AU - Kim, Yul Ho

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AB - Rice stripe virus (RSV) is a viral disease that seriously impacts rice production in East Asia, most notably in Korea, China, and Japan. Highly RSV-resistant transgenic japonica rice plants were generated using a dsRNAi construct designed to silence the entire sequence region of the RSV-CP gene. Transgenic rice plants were inoculated with a population of viruliferous insects, small brown planthoppers (SBPH), and their resistance was evaluated using ELISA and an infection rate assay. A correlation between the expression of the RSV-CP homologous small RNAs and the RSV resistance of the transgenic rice lines was discovered. These plants were also analyzed by comparing the expression pattern of invading viral genes, small RNA production and the stable transmission of the RSV resistance trait to the T3 generation. Furthermore, the agronomic trait was stably transmitted to the T4 generation of transgenic plants.

KW - Movement protein (MP)

KW - Oryza sativa L.

KW - Rice stripe virus (RSV)

KW - RNAi

KW - RSV coat protein

KW - Silencing suppressor (NS3)

KW - Small brown planthopper (SBPH)

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SN - 1016-8478

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JO - Molecules and Cells

JF - Molecules and Cells

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ER -

Suppression of NS3 and MP is important for the stable inheritance of RNAi-mediated rice stripe virus (RSV) resistance obtained by targeting the fully complementary RSV-CP gene

Abstract

Keywords

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