Suppressive effects of (-)-tubaic acid on RANKL-induced osteoclast differentiation and bone resorption

Soomin Lim, Hye Jung Ihn, Ju Ang Kim, Jong Sup Bae, Jung Eun Kim, Yong Chul Bae, Hong In Shin, Tae Hoon Kim, Eui Kyun Park

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Regulation of osteoclastogenesis and bone-resorbing activity can be an efficacious strategy for treating bone loss diseases because excessive osteoclastic bone resorption leads to the development of such diseases. Here, we investigated the role of (-)-tubaic acid, a thermal degradation product of rotenone, in osteoclast formation and function in an attempt to identify alternative natural compounds. (-)-Tubaic acid significantly inhibited receptor activator of nuclear factor-κB ligand (RANKL)-mediated osteoclast differentiation at both the early and late stages, suggesting that (-)-tubaic acid affects the commitment and differentiation of osteoclast progenitors as well as the cell-cell fusion of mononuclear osteoclasts. (-)-Tubaic acid attenuated the activation of extracellular signal-regulated kinase (ERK) and expression of nuclear factor of activated T-cells cytoplasmic 1 (NFATc1) and its target genes in response to RANKL. Furthermore, a pit-formation assay revealed that (-)-tubaic acid significantly impaired the bone-resorbing activity of osteoclasts. Our results demonstrated that (-)-tubaic acid exhibits anti-osteoclastogenic and anti-resorptive effects, indicating its therapeutic potential in the management of osteoclast-related bone diseases.

Original languageEnglish
Pages (from-to)1-9
Number of pages9
JournalAnimal Cells and Systems
Volume27
Issue number1
DOIs
StatePublished - 2023

Keywords

  • (-)-tubaic acid
  • bone resorption
  • nuclear factor of activated T-cells cytoplasmic 1 (NFATc1)
  • osteoclast

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