Surface grafting of glycidyl methacrylate on silica gel and polyethylene beads

Seong Ho Choi; Young Mi Hwang; Jae Jeong Ryoo; Kwang Pill Lee; Kazutoku Ohta; Toyohide Takeuchi; Ji Ye Jin; Chuzo Fujimoto

doi:10.1002/elps.200305581

Surface grafting of glycidyl methacrylate on silica gel and polyethylene beads

Seong Ho Choi, Young Mi Hwang, Jae Jeong Ryoo, Kwang Pill Lee, Kazutoku Ohta, Toyohide Takeuchi, Ji Ye Jin, Chuzo Fujimoto

Department of Chemical Science Education

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Surface grafting of glycidyl methacrylate (GMA) on silica gel and a polyethylene bead was performed by radical polymerization and radiation-induced polymerization, respectively, in order to improve softness. Subsequently, diethylene triamine (DETA), triethylene tetraamine (TETA), and iminodiacetic acid (IDA) were introduced to the grafted GMA for use as affinity columns. The efficiency of the affinity column was investigated by use of bovine serum albumin (BSA) and hemoglobin (Hb) as model proteins. The affinity degree of BSA was higher than Hb for the DETA and TTA column, whereas the affinity degree of Hb was higher than BSA for the IDA column supported by silica gel. The affinity degree of BSA was higher than Hb for the DETA and TTA column supported by polyethylene (PE) beads.

Original language	English
Pages (from-to)	3181-3186
Number of pages	6
Journal	Electrophoresis
Volume	24
Issue number	18
DOIs	https://doi.org/10.1002/elps.200305581
State	Published - Sep 2003

Keywords

Affinity column
Bovine serum albumin
Efficiency
Glycidyl methacrylate
Hemoglobin
Surface grafting

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10.1002/elps.200305581

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title = "Surface grafting of glycidyl methacrylate on silica gel and polyethylene beads",

abstract = "Surface grafting of glycidyl methacrylate (GMA) on silica gel and a polyethylene bead was performed by radical polymerization and radiation-induced polymerization, respectively, in order to improve softness. Subsequently, diethylene triamine (DETA), triethylene tetraamine (TETA), and iminodiacetic acid (IDA) were introduced to the grafted GMA for use as affinity columns. The efficiency of the affinity column was investigated by use of bovine serum albumin (BSA) and hemoglobin (Hb) as model proteins. The affinity degree of BSA was higher than Hb for the DETA and TTA column, whereas the affinity degree of Hb was higher than BSA for the IDA column supported by silica gel. The affinity degree of BSA was higher than Hb for the DETA and TTA column supported by polyethylene (PE) beads.",

keywords = "Affinity column, Bovine serum albumin, Efficiency, Glycidyl methacrylate, Hemoglobin, Surface grafting",

author = "Choi, {Seong Ho} and Hwang, {Young Mi} and Ryoo, {Jae Jeong} and Lee, {Kwang Pill} and Kazutoku Ohta and Toyohide Takeuchi and Jin, {Ji Ye} and Chuzo Fujimoto",

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T1 - Surface grafting of glycidyl methacrylate on silica gel and polyethylene beads

AU - Choi, Seong Ho

AU - Hwang, Young Mi

AU - Ryoo, Jae Jeong

AU - Lee, Kwang Pill

AU - Ohta, Kazutoku

AU - Takeuchi, Toyohide

AU - Jin, Ji Ye

AU - Fujimoto, Chuzo

PY - 2003/9

Y1 - 2003/9

N2 - Surface grafting of glycidyl methacrylate (GMA) on silica gel and a polyethylene bead was performed by radical polymerization and radiation-induced polymerization, respectively, in order to improve softness. Subsequently, diethylene triamine (DETA), triethylene tetraamine (TETA), and iminodiacetic acid (IDA) were introduced to the grafted GMA for use as affinity columns. The efficiency of the affinity column was investigated by use of bovine serum albumin (BSA) and hemoglobin (Hb) as model proteins. The affinity degree of BSA was higher than Hb for the DETA and TTA column, whereas the affinity degree of Hb was higher than BSA for the IDA column supported by silica gel. The affinity degree of BSA was higher than Hb for the DETA and TTA column supported by polyethylene (PE) beads.

AB - Surface grafting of glycidyl methacrylate (GMA) on silica gel and a polyethylene bead was performed by radical polymerization and radiation-induced polymerization, respectively, in order to improve softness. Subsequently, diethylene triamine (DETA), triethylene tetraamine (TETA), and iminodiacetic acid (IDA) were introduced to the grafted GMA for use as affinity columns. The efficiency of the affinity column was investigated by use of bovine serum albumin (BSA) and hemoglobin (Hb) as model proteins. The affinity degree of BSA was higher than Hb for the DETA and TTA column, whereas the affinity degree of Hb was higher than BSA for the IDA column supported by silica gel. The affinity degree of BSA was higher than Hb for the DETA and TTA column supported by polyethylene (PE) beads.

KW - Affinity column

KW - Bovine serum albumin

KW - Efficiency

KW - Glycidyl methacrylate

KW - Hemoglobin

KW - Surface grafting

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Surface grafting of glycidyl methacrylate on silica gel and polyethylene beads

Abstract

Keywords

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