Surfactin blocks NO production in lipopolysaccharide-activated macrophages by inhibiting NF-κB activation

Se Eun Byeon; Yong Gyu Lee; Byung Hun Kim; Ting Shen; Sang Yeol Lee; Hwa Jin Park; Seung Chun Park; Man Hee Rhee; Jae Youl Cho

doi:10.4014/jmb.0800.189

Surfactin blocks NO production in lipopolysaccharide-activated macrophages by inhibiting NF-κB activation

Se Eun Byeon, Yong Gyu Lee, Byung Hun Kim, Ting Shen, Sang Yeol Lee, Hwa Jin Park, Seung Chun Park, Man Hee Rhee, Jae Youl Cho

Department of Veterinary Medicine

Research output: Contribution to journal › Article › peer-review

65 Scopus citations

Abstract

Surfactin is a natural biosurfactant derived from Bacillus subtilis and has various biological activities such as anticancer, antiplatelet, and anti-inflammatory effects. In this study, the inhibitory mechanism of surfactin in NO production from macrophages was examined. Surfactin downregulated LPS-induced NO production in RAW264.7 cells and primary macrophages with IC₅₀ values of 31.6 and 22.4 μM, respectively. Immunoblotting analysis showed that surfactin strongly blocked the phosphorylation of IKK and IκBα and the nuclear translocation of NF-κB (p65). Therefore, these data suggest that surfactin may act as a bacterium-derived anti-inflammatory agent with anti-NF-κB activity.

Original language	English
Pages (from-to)	1984-1989
Number of pages	6
Journal	Journal of Microbiology and Biotechnology
Volume	18
Issue number	12
DOIs	https://doi.org/10.4014/jmb.0800.189
State	Published - 28 Dec 2008

Keywords

Anti-inflammatory effect
Bacillus subtilis
Lipopolysaccharide
NF-κB
Nitric oxide
Surfactin

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10.4014/jmb.0800.189

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abstract = "Surfactin is a natural biosurfactant derived from Bacillus subtilis and has various biological activities such as anticancer, antiplatelet, and anti-inflammatory effects. In this study, the inhibitory mechanism of surfactin in NO production from macrophages was examined. Surfactin downregulated LPS-induced NO production in RAW264.7 cells and primary macrophages with IC50 values of 31.6 and 22.4 μM, respectively. Immunoblotting analysis showed that surfactin strongly blocked the phosphorylation of IKK and IκBα and the nuclear translocation of NF-κB (p65). Therefore, these data suggest that surfactin may act as a bacterium-derived anti-inflammatory agent with anti-NF-κB activity.",

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AU - Byeon, Se Eun

AU - Lee, Yong Gyu

AU - Kim, Byung Hun

AU - Shen, Ting

AU - Lee, Sang Yeol

AU - Park, Hwa Jin

AU - Park, Seung Chun

AU - Rhee, Man Hee

AU - Cho, Jae Youl

PY - 2008/12/28

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AB - Surfactin is a natural biosurfactant derived from Bacillus subtilis and has various biological activities such as anticancer, antiplatelet, and anti-inflammatory effects. In this study, the inhibitory mechanism of surfactin in NO production from macrophages was examined. Surfactin downregulated LPS-induced NO production in RAW264.7 cells and primary macrophages with IC50 values of 31.6 and 22.4 μM, respectively. Immunoblotting analysis showed that surfactin strongly blocked the phosphorylation of IKK and IκBα and the nuclear translocation of NF-κB (p65). Therefore, these data suggest that surfactin may act as a bacterium-derived anti-inflammatory agent with anti-NF-κB activity.

KW - Anti-inflammatory effect

KW - Bacillus subtilis

KW - Lipopolysaccharide

KW - NF-κB

KW - Nitric oxide

KW - Surfactin

UR - https://www.scopus.com/pages/publications/67349279544

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DO - 10.4014/jmb.0800.189

M3 - Article

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AN - SCOPUS:67349279544

SN - 1017-7825

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SP - 1984

EP - 1989

JO - Journal of Microbiology and Biotechnology

JF - Journal of Microbiology and Biotechnology

IS - 12

ER -

Surfactin blocks NO production in lipopolysaccharide-activated macrophages by inhibiting NF-κB activation

Abstract

Keywords

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