Sustainable Production of Shinorine from Lignocellulosic Biomass by Metabolically Engineered Saccharomyces cerevisiae

So Rim Kim, Minseok Cha, Taeok Kim, Sihoon Song, Hye Jee Kang, Younghoon Jung, Jeong Yong Cho, Sang Hyun Moh, Soo Jung Kim

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Mycosporine-like amino acids (MAAs) have been used in cosmetics and pharmaceuticals. The purpose of this work was to develop yeast strains for sustainable and economical production of MAAs, especially shinorine. First, genes involved in MAA biosynthetic pathway from Actinosynnema mirum were introduced into Saccharomyces cerevisiae for heterologous shinorine production. Second, combinatorial expression of wild and mutant xylose reductase was adopted in the engineered S. cerevisiae to facilitate xylose utilization in the pentose phosphate pathway. Finally, the accumulation of sedoheptulose 7-phosphate (S7P) was attempted by deleting transaldolase-encoding TAL1 in the pentose phosphate pathway to increase carbon flux toward shinorine production. In fed-batch fermentation, the engineered strain (DXdT-M) produced 751 mg/L shinorine in 71 h. Ultimately, 54 mg/L MAAs was produced by DXdT-M from rice straw hydrolysate. The results suggest that shinorine production by S. cerevisiae might be a promising process for sustainable production and industrial applications.

Original languageEnglish
Pages (from-to)15848-15858
Number of pages11
JournalJournal of Agricultural and Food Chemistry
Volume70
Issue number50
DOIs
StatePublished - 21 Dec 2022

Keywords

  • lignocellulose
  • mycosporine-like amino acids (MAAs)
  • Saccharomyces cerevisiae
  • shinorine

Fingerprint

Dive into the research topics of 'Sustainable Production of Shinorine from Lignocellulosic Biomass by Metabolically Engineered Saccharomyces cerevisiae'. Together they form a unique fingerprint.

Cite this