Abstract
The detection of image is important to vision. Direction-selective retinal ganglion cells (DS-RGCs) respond strongly to stimuli moving in one direction of motion and are strongly inhibited by stimuli moving in the opposite direction. In this article, we investigated the distributions of kainate glutamate receptor subtypes KA1 and KA2 on the dendritic arbors of DS-RGCs in developing (5, 10) days postnatal (PN) and adult mouse retina to search for anisotropies. The distribution of kainate receptor subtypes on the DS-RGCs was determined using antibody immunocytochemistry. To identify their characteristic morphology, DS-RGCs were injected with Lucifer yellow. The triple-labeled images of dendrites, kinesin II, and receptors were visualized by confocal microscopy and were reconstructed from high-resolution confocal images. We found no evidence of asymmetry in any of the kainate receptor subunits examined on the dendritic arbors of both the On and Off layers of DS-RGCs in all periods of developing and adult stage that would predict direction selectivity.
Original language | English |
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Pages (from-to) | 35-45 |
Number of pages | 11 |
Journal | Acta Histochemica et Cytochemica |
Volume | 45 |
Issue number | 1 |
DOIs | |
State | Published - 2012 |
Keywords
- Cell injection
- Direction selectivity
- Immunocytochemistry
- Kainate glutamate receptor
- Retinal ganglion cell