Synergistic effect of ROS-generating polydopamine on drug-induced bone tissue regeneration

Hyeju Han, Bongkyun Kang, Shazid Md Sharker, Tabassum Binte Kashem, Yuejin Kim, Jeehee Lee, Minok Park, Eunjeong Kim, Yun Jae Jung, Jinkyu Lim, Seungwon Ryu, Kyueui Lee

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

A PHD (prolyl hydroxylase) inhibitor, 1,4-dihydrophenonthrolin-4-one-3-carboxylic acid (1,4-DPCA), is a drug that can artificially promote tissue regeneration by enhancing metabolic activity through the upregulation of hypoxia inducible factor 1 subunit alpha (Hif-1α) under normoxic conditions. This study presents a novel design methodology for a drug delivery system to maximize the regenerative effect of 1,4-DPCA. Specifically, by encapsulating 1,4-DPCA in polydopamine (PDA) that generates reactive oxygen species (ROS), the combined effects of Hif-1α upregulation and the induction of cellular antioxidant defense mechanisms by localized ROS can significantly enhance tissue regeneration. The study confirmed that each material (PDA and 1,4-DPCA) triggers a positive synergistic effect on the regenerative mechanisms. As a result, the use of a PDA drug delivery system loaded with 1,4-DPCA showed approximately six times greater bone regeneration compared to the control (no treatment) in a mouse calvarial defect model.

Original languageEnglish
Pages (from-to)20118-20130
Number of pages13
JournalNanoscale
Volume16
Issue number43
DOIs
StatePublished - 10 Oct 2024

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