Synthesis and validation of novel cholesterol-based fluorescent lipids designed to observe the cellular trafficking of cationic liposomes

Bieong Kil Kim; Young Bae Seu; Jong Soo Choi; Jong Won Park; Kyung Oh Doh

doi:10.1016/j.bmcl.2015.07.051

Synthesis and validation of novel cholesterol-based fluorescent lipids designed to observe the cellular trafficking of cationic liposomes

Bieong Kil Kim, Young Bae Seu, Jong Soo Choi, Jong Won Park, Kyung Oh Doh

School of Life Science and Biotechnology

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Abstract Cholesterol-based fluorescent lipids with ether linker were synthesized using NBD (Chol-E-NBD) or Rhodamine B (Chol-E-Rh), and the usefulnesses as fluorescent probes for tracing cholesterol-based liposomes were validated. The fluorescent intensities of liposomes containing these modified lipids were measured and observed under a microscope. Neither compound interfered with the expression of GFP plasmid, and live cell images were obtained without interferences. Changes in the fluorescent intensity of liposomes containing Chol-E-NBD were followed by flow cytometry for up to 24 h. These fluorescent lipids could be useful probes for trafficking of cationic liposome-mediated gene delivery.

Original language	English
Article number	22940
Pages (from-to)	3893-3896
Number of pages	4
Journal	Bioorganic and Medicinal Chemistry Letters
Volume	25
Issue number	18
DOIs	https://doi.org/10.1016/j.bmcl.2015.07.051
State	Published - 17 Aug 2015

Keywords

Cholesterol
Fluorescent probe
Liposome
Transfection

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10.1016/j.bmcl.2015.07.051

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title = "Synthesis and validation of novel cholesterol-based fluorescent lipids designed to observe the cellular trafficking of cationic liposomes",

abstract = "Abstract Cholesterol-based fluorescent lipids with ether linker were synthesized using NBD (Chol-E-NBD) or Rhodamine B (Chol-E-Rh), and the usefulnesses as fluorescent probes for tracing cholesterol-based liposomes were validated. The fluorescent intensities of liposomes containing these modified lipids were measured and observed under a microscope. Neither compound interfered with the expression of GFP plasmid, and live cell images were obtained without interferences. Changes in the fluorescent intensity of liposomes containing Chol-E-NBD were followed by flow cytometry for up to 24 h. These fluorescent lipids could be useful probes for trafficking of cationic liposome-mediated gene delivery.",

keywords = "Cholesterol, Fluorescent probe, Liposome, Transfection",

author = "Kim, {Bieong Kil} and Seu, {Young Bae} and Choi, {Jong Soo} and Park, {Jong Won} and Doh, {Kyung Oh}",

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doi = "10.1016/j.bmcl.2015.07.051",

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T1 - Synthesis and validation of novel cholesterol-based fluorescent lipids designed to observe the cellular trafficking of cationic liposomes

AU - Kim, Bieong Kil

AU - Seu, Young Bae

AU - Choi, Jong Soo

AU - Park, Jong Won

AU - Doh, Kyung Oh

PY - 2015/8/17

Y1 - 2015/8/17

N2 - Abstract Cholesterol-based fluorescent lipids with ether linker were synthesized using NBD (Chol-E-NBD) or Rhodamine B (Chol-E-Rh), and the usefulnesses as fluorescent probes for tracing cholesterol-based liposomes were validated. The fluorescent intensities of liposomes containing these modified lipids were measured and observed under a microscope. Neither compound interfered with the expression of GFP plasmid, and live cell images were obtained without interferences. Changes in the fluorescent intensity of liposomes containing Chol-E-NBD were followed by flow cytometry for up to 24 h. These fluorescent lipids could be useful probes for trafficking of cationic liposome-mediated gene delivery.

AB - Abstract Cholesterol-based fluorescent lipids with ether linker were synthesized using NBD (Chol-E-NBD) or Rhodamine B (Chol-E-Rh), and the usefulnesses as fluorescent probes for tracing cholesterol-based liposomes were validated. The fluorescent intensities of liposomes containing these modified lipids were measured and observed under a microscope. Neither compound interfered with the expression of GFP plasmid, and live cell images were obtained without interferences. Changes in the fluorescent intensity of liposomes containing Chol-E-NBD were followed by flow cytometry for up to 24 h. These fluorescent lipids could be useful probes for trafficking of cationic liposome-mediated gene delivery.

KW - Cholesterol

KW - Fluorescent probe

KW - Liposome

KW - Transfection

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DO - 10.1016/j.bmcl.2015.07.051

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AN - SCOPUS:84939261345

SN - 0960-894X

VL - 25

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JO - Bioorganic and Medicinal Chemistry Letters

JF - Bioorganic and Medicinal Chemistry Letters

IS - 18

M1 - 22940

ER -

Synthesis and validation of novel cholesterol-based fluorescent lipids designed to observe the cellular trafficking of cationic liposomes

Abstract

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