Synthesis and validation of novel cholesterol-based fluorescent lipids designed to observe the cellular trafficking of cationic liposomes

Bieong Kil Kim; Young Bae Seu; Jong Soo Choi; Jong Won Park; Kyung Oh Doh

doi:10.1016/j.bmcl.2015.07.051

Synthesis and validation of novel cholesterol-based fluorescent lipids designed to observe the cellular trafficking of cationic liposomes

Bieong Kil Kim
, Young Bae Seu
, Jong Soo Choi
, Jong Won Park
, Kyung Oh Doh

School of Life Science and Biotechnology

Kyungpook National University
Yeungnam University

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Abstract Cholesterol-based fluorescent lipids with ether linker were synthesized using NBD (Chol-E-NBD) or Rhodamine B (Chol-E-Rh), and the usefulnesses as fluorescent probes for tracing cholesterol-based liposomes were validated. The fluorescent intensities of liposomes containing these modified lipids were measured and observed under a microscope. Neither compound interfered with the expression of GFP plasmid, and live cell images were obtained without interferences. Changes in the fluorescent intensity of liposomes containing Chol-E-NBD were followed by flow cytometry for up to 24 h. These fluorescent lipids could be useful probes for trafficking of cationic liposome-mediated gene delivery.

Original language	English
Article number	22940
Pages (from-to)	3893-3896
Number of pages	4
Journal	Bioorganic and Medicinal Chemistry Letters
Volume	25
Issue number	18
DOIs	https://doi.org/10.1016/j.bmcl.2015.07.051
State	Published - 17 Aug 2015

Keywords

Cholesterol
Fluorescent probe
Liposome
Transfection

Access to Document

10.1016/j.bmcl.2015.07.051

Cite this

@article{03a4db31934f40d983a33510e4bf5145,

title = "Synthesis and validation of novel cholesterol-based fluorescent lipids designed to observe the cellular trafficking of cationic liposomes",

abstract = "Abstract Cholesterol-based fluorescent lipids with ether linker were synthesized using NBD (Chol-E-NBD) or Rhodamine B (Chol-E-Rh), and the usefulnesses as fluorescent probes for tracing cholesterol-based liposomes were validated. The fluorescent intensities of liposomes containing these modified lipids were measured and observed under a microscope. Neither compound interfered with the expression of GFP plasmid, and live cell images were obtained without interferences. Changes in the fluorescent intensity of liposomes containing Chol-E-NBD were followed by flow cytometry for up to 24 h. These fluorescent lipids could be useful probes for trafficking of cationic liposome-mediated gene delivery.",

keywords = "Cholesterol, Fluorescent probe, Liposome, Transfection",

author = "Kim, \{Bieong Kil\} and Seu, \{Young Bae\} and Choi, \{Jong Soo\} and Park, \{Jong Won\} and Doh, \{Kyung Oh\}",

note = "Publisher Copyright: {\textcopyright} 2015 Elsevier Ltd.",

year = "2015",

month = aug,

day = "17",

doi = "10.1016/j.bmcl.2015.07.051",

language = "English",

volume = "25",

pages = "3893--3896",

journal = "Bioorganic and Medicinal Chemistry Letters",

issn = "0960-894X",

publisher = "Elsevier Ltd.",

number = "18",

}

TY - JOUR

T1 - Synthesis and validation of novel cholesterol-based fluorescent lipids designed to observe the cellular trafficking of cationic liposomes

AU - Kim, Bieong Kil

AU - Seu, Young Bae

AU - Choi, Jong Soo

AU - Park, Jong Won

AU - Doh, Kyung Oh

PY - 2015/8/17

Y1 - 2015/8/17

N2 - Abstract Cholesterol-based fluorescent lipids with ether linker were synthesized using NBD (Chol-E-NBD) or Rhodamine B (Chol-E-Rh), and the usefulnesses as fluorescent probes for tracing cholesterol-based liposomes were validated. The fluorescent intensities of liposomes containing these modified lipids were measured and observed under a microscope. Neither compound interfered with the expression of GFP plasmid, and live cell images were obtained without interferences. Changes in the fluorescent intensity of liposomes containing Chol-E-NBD were followed by flow cytometry for up to 24 h. These fluorescent lipids could be useful probes for trafficking of cationic liposome-mediated gene delivery.

AB - Abstract Cholesterol-based fluorescent lipids with ether linker were synthesized using NBD (Chol-E-NBD) or Rhodamine B (Chol-E-Rh), and the usefulnesses as fluorescent probes for tracing cholesterol-based liposomes were validated. The fluorescent intensities of liposomes containing these modified lipids were measured and observed under a microscope. Neither compound interfered with the expression of GFP plasmid, and live cell images were obtained without interferences. Changes in the fluorescent intensity of liposomes containing Chol-E-NBD were followed by flow cytometry for up to 24 h. These fluorescent lipids could be useful probes for trafficking of cationic liposome-mediated gene delivery.

KW - Cholesterol

KW - Fluorescent probe

KW - Liposome

KW - Transfection

UR - https://www.scopus.com/pages/publications/84939261345

U2 - 10.1016/j.bmcl.2015.07.051

DO - 10.1016/j.bmcl.2015.07.051

M3 - Article

C2 - 26243368

AN - SCOPUS:84939261345

SN - 0960-894X

VL - 25

SP - 3893

EP - 3896

JO - Bioorganic and Medicinal Chemistry Letters

JF - Bioorganic and Medicinal Chemistry Letters

IS - 18

M1 - 22940

ER -

Synthesis and validation of novel cholesterol-based fluorescent lipids designed to observe the cellular trafficking of cationic liposomes

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this