TY - JOUR
T1 - Synthesis, Characterization, and Enhanced Cancer-Imaging Application of Trans-activator of Transcription Peptide-conjugated Ultrasmall Gadolinium Oxide Nanoparticles
AU - Ahmad, Mohammad Yaseen
AU - Cha, Hyunsil
AU - Oh, In Taek
AU - Tegafaw, Tirusew
AU - Miao, Xu
AU - Ho, Son Long
AU - Marasini, Shanti
AU - Ghazanfari, Adibehalsadat
AU - Yue, Huan
AU - Chae, Kwon Seok
AU - Chang, Yongmin
AU - Lee, Gang Ho
N1 - Publisher Copyright:
© 2018 Korean Chemical Society, Seoul & Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim
PY - 2018/4
Y1 - 2018/4
N2 - We prepared gadolinium oxide (Gd2O3) nanoparticles (GNPs) coated with a trans-activator of transcription (TAT) peptide with cell-penetrating ability (i.e., TAT-GNPs) through one-pot process. We characterized the particle diameter, surface-coating structure, water proton relaxivities, and in vitro cellular toxicities of the TAT-GNPs. We measured in vivo T1 magnetic resonance images (MRI) in a model nude mouse with liver cancer prior and posterior to intravenous administration. The average particle diameter of the GNPs was 1.5 nm. The sample solution exhibited a longitudinal water proton relaxivity (r1) of 18.2/s/mM (r2/r1 = 1.6, r2 = transverse water proton relaxivity), which is four to five times higher than those of commercial Gd-chelates. The in vivo T1 MRI exhibited positively (or T1) enhanced contrasts in the mouse liver cancer after intravenous administration, demonstrating that the TAT-GNPs acted as an enhanced cancer-imaging agent similar to the cancer-targeting agent in T1 MRI.
AB - We prepared gadolinium oxide (Gd2O3) nanoparticles (GNPs) coated with a trans-activator of transcription (TAT) peptide with cell-penetrating ability (i.e., TAT-GNPs) through one-pot process. We characterized the particle diameter, surface-coating structure, water proton relaxivities, and in vitro cellular toxicities of the TAT-GNPs. We measured in vivo T1 magnetic resonance images (MRI) in a model nude mouse with liver cancer prior and posterior to intravenous administration. The average particle diameter of the GNPs was 1.5 nm. The sample solution exhibited a longitudinal water proton relaxivity (r1) of 18.2/s/mM (r2/r1 = 1.6, r2 = transverse water proton relaxivity), which is four to five times higher than those of commercial Gd-chelates. The in vivo T1 MRI exhibited positively (or T1) enhanced contrasts in the mouse liver cancer after intravenous administration, demonstrating that the TAT-GNPs acted as an enhanced cancer-imaging agent similar to the cancer-targeting agent in T1 MRI.
KW - Enhanced cancer imaging
KW - T magnetic resonance imaging
KW - Trans-activator of transcription peptide
KW - Trans-activator of transcription-gadolinium oxide nanoparticles
KW - Ultrasmall gadolinium oxide nanoparticles
UR - http://www.scopus.com/inward/record.url?scp=85042591656&partnerID=8YFLogxK
U2 - 10.1002/bkcs.11404
DO - 10.1002/bkcs.11404
M3 - Article
AN - SCOPUS:85042591656
SN - 0253-2964
VL - 39
SP - 435
EP - 441
JO - Bulletin of the Korean Chemical Society
JF - Bulletin of the Korean Chemical Society
IS - 4
ER -