Synthesis of silver nanoparticles using Leucas biflora (Vahl) Sm. Leaf extracts and their activity on breast cancer (MDA-MB-231) cells

The goal of this study is to develop the Leucas biflora (Vahl)Sm. leaf extract fabricated silver nanoparticles (LbLE-Ag-NPs) as a potential anticancer agent using a simple green chemistry approach. Synthesized LbLE-AgNPs were found to be hexagonal shape, crystalline nature with a size range of 40–98 nm, and have zeta potential at −0.138 mV, according to the analytical data. The functional groups bonded with LbLE-Ag-NPs were screened as C-Br, C-Cl, OH, C-O-C, and C = C, etc. LbLE-AgNPs exhibited an effective dose-dependent anti-cancerous activity on MDA-MB-231 (breast cancer cell line) and the minimum inhibitory concentration (IC₅₀) value is measured as 95 ± 0.21 µg/ml. The induction of apoptosis was also confirmed by fluorescence-based staining upon treatment. The resultant LbLE-Ag-NPs could be used in the anticancer progress as an alternate agent after the detailed scrutinize.