Targeting of membrane type1-matrix metalloproteinase (MT1-MMP) using superparamagnetic nanoparticles in human liver cancer cells

Bo Hyung Park; Yongmin Chang; Young Ju Lee; Ji Ae Park; In Sung Kim; Sung Jin Bae; Gang Ho Lee; Tae Jeong Kim; Jong Yeol Kim; Hye Jung Kim; Jae Chang Jung

doi:10.1016/j.colsurfa.2007.04.171

Targeting of membrane type1-matrix metalloproteinase (MT1-MMP) using superparamagnetic nanoparticles in human liver cancer cells

Bo Hyung Park, Yongmin Chang, Young Ju Lee, Ji Ae Park, In Sung Kim, Sung Jin Bae, Gang Ho Lee, Tae Jeong Kim, Jong Yeol Kim, Hye Jung Kim, Jae Chang Jung

Kyungpook National University

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Magnetic nanoparticles in colloid form are attractive materials that have been widely exploited in medicine for diagnostic imaging and therapeutic applications. Iron oxide nanoparticle functionalized with molecular marker is a new class of imaging agent to visualize the specific molecular event inside living animal or human. MT1-MMP plays a critical role in tumor progression and metastasis and has become a promising diagnostic cell surface marker. Therefore, our purpose was to obtain the MR image of MT1-MMP in cancer bearing nude mice using biotinylated monoclonal anti-MT1-MMP antibody conjugated streptavidinylated ultrasmall superparamagnetic iron oxides (USPIOs) (MT1-MMP-USPIOs). The results showed strong MR contrast change in the human liver cancer (SK-HEP-1 cells) but not in the human colon cancer (HT-29 cells) after intravenous injection of MT1-MMP-USPIOs. Furthermore, Prussian Blue staining assessed the internalized MT1-MMP-USPIOs into the SK-HEP-1 derived tumor cells. Taken together, our results suggest that the targeting of an early diagnosis of human liver cancer (SK-HEP-1 cells) is feasible by using MT1-MMP-USPIOs.

Original language	English
Pages (from-to)	647-650
Number of pages	4
Journal	Colloids and Surfaces A: Physicochemical and Engineering Aspects
Volume	313-314
DOIs	https://doi.org/10.1016/j.colsurfa.2007.04.171
State	Published - 1 Feb 2008

Keywords

Membrane type1-matrix metalloproteinase (MT1-MMP)
Molecular imaging
MRI
Nude mouse
Tumor

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.colsurfa.2007.04.171

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Park, B. H., Chang, Y., Lee, Y. J., Park, J. A., Kim, I. S., Bae, S. J., Lee, G. H., Kim, T. J., Kim, J. Y., Kim, H. J., & Jung, J. C. (2008). Targeting of membrane type1-matrix metalloproteinase (MT1-MMP) using superparamagnetic nanoparticles in human liver cancer cells. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 313-314, 647-650. https://doi.org/10.1016/j.colsurfa.2007.04.171

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title = "Targeting of membrane type1-matrix metalloproteinase (MT1-MMP) using superparamagnetic nanoparticles in human liver cancer cells",

abstract = "Magnetic nanoparticles in colloid form are attractive materials that have been widely exploited in medicine for diagnostic imaging and therapeutic applications. Iron oxide nanoparticle functionalized with molecular marker is a new class of imaging agent to visualize the specific molecular event inside living animal or human. MT1-MMP plays a critical role in tumor progression and metastasis and has become a promising diagnostic cell surface marker. Therefore, our purpose was to obtain the MR image of MT1-MMP in cancer bearing nude mice using biotinylated monoclonal anti-MT1-MMP antibody conjugated streptavidinylated ultrasmall superparamagnetic iron oxides (USPIOs) (MT1-MMP-USPIOs). The results showed strong MR contrast change in the human liver cancer (SK-HEP-1 cells) but not in the human colon cancer (HT-29 cells) after intravenous injection of MT1-MMP-USPIOs. Furthermore, Prussian Blue staining assessed the internalized MT1-MMP-USPIOs into the SK-HEP-1 derived tumor cells. Taken together, our results suggest that the targeting of an early diagnosis of human liver cancer (SK-HEP-1 cells) is feasible by using MT1-MMP-USPIOs.",

keywords = "Membrane type1-matrix metalloproteinase (MT1-MMP), Molecular imaging, MRI, Nude mouse, Tumor",

author = "Park, {Bo Hyung} and Yongmin Chang and Lee, {Young Ju} and Park, {Ji Ae} and Kim, {In Sung} and Bae, {Sung Jin} and Lee, {Gang Ho} and Kim, {Tae Jeong} and Kim, {Jong Yeol} and Kim, {Hye Jung} and Jung, {Jae Chang}",

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Park, BH, Chang, Y, Lee, YJ, Park, JA, Kim, IS, Bae, SJ, Lee, GH, Kim, TJ, Kim, JY, Kim, HJ & Jung, JC 2008, 'Targeting of membrane type1-matrix metalloproteinase (MT1-MMP) using superparamagnetic nanoparticles in human liver cancer cells', Colloids and Surfaces A: Physicochemical and Engineering Aspects, vol. 313-314, pp. 647-650. https://doi.org/10.1016/j.colsurfa.2007.04.171

TY - JOUR

T1 - Targeting of membrane type1-matrix metalloproteinase (MT1-MMP) using superparamagnetic nanoparticles in human liver cancer cells

AU - Park, Bo Hyung

AU - Chang, Yongmin

AU - Lee, Young Ju

AU - Park, Ji Ae

AU - Kim, In Sung

AU - Bae, Sung Jin

AU - Lee, Gang Ho

AU - Kim, Tae Jeong

AU - Kim, Jong Yeol

AU - Kim, Hye Jung

AU - Jung, Jae Chang

PY - 2008/2/1

Y1 - 2008/2/1

N2 - Magnetic nanoparticles in colloid form are attractive materials that have been widely exploited in medicine for diagnostic imaging and therapeutic applications. Iron oxide nanoparticle functionalized with molecular marker is a new class of imaging agent to visualize the specific molecular event inside living animal or human. MT1-MMP plays a critical role in tumor progression and metastasis and has become a promising diagnostic cell surface marker. Therefore, our purpose was to obtain the MR image of MT1-MMP in cancer bearing nude mice using biotinylated monoclonal anti-MT1-MMP antibody conjugated streptavidinylated ultrasmall superparamagnetic iron oxides (USPIOs) (MT1-MMP-USPIOs). The results showed strong MR contrast change in the human liver cancer (SK-HEP-1 cells) but not in the human colon cancer (HT-29 cells) after intravenous injection of MT1-MMP-USPIOs. Furthermore, Prussian Blue staining assessed the internalized MT1-MMP-USPIOs into the SK-HEP-1 derived tumor cells. Taken together, our results suggest that the targeting of an early diagnosis of human liver cancer (SK-HEP-1 cells) is feasible by using MT1-MMP-USPIOs.

AB - Magnetic nanoparticles in colloid form are attractive materials that have been widely exploited in medicine for diagnostic imaging and therapeutic applications. Iron oxide nanoparticle functionalized with molecular marker is a new class of imaging agent to visualize the specific molecular event inside living animal or human. MT1-MMP plays a critical role in tumor progression and metastasis and has become a promising diagnostic cell surface marker. Therefore, our purpose was to obtain the MR image of MT1-MMP in cancer bearing nude mice using biotinylated monoclonal anti-MT1-MMP antibody conjugated streptavidinylated ultrasmall superparamagnetic iron oxides (USPIOs) (MT1-MMP-USPIOs). The results showed strong MR contrast change in the human liver cancer (SK-HEP-1 cells) but not in the human colon cancer (HT-29 cells) after intravenous injection of MT1-MMP-USPIOs. Furthermore, Prussian Blue staining assessed the internalized MT1-MMP-USPIOs into the SK-HEP-1 derived tumor cells. Taken together, our results suggest that the targeting of an early diagnosis of human liver cancer (SK-HEP-1 cells) is feasible by using MT1-MMP-USPIOs.

KW - Membrane type1-matrix metalloproteinase (MT1-MMP)

KW - Molecular imaging

KW - MRI

KW - Nude mouse

KW - Tumor

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JO - Colloids and Surfaces A: Physicochemical and Engineering Aspects

JF - Colloids and Surfaces A: Physicochemical and Engineering Aspects

ER -

Targeting of membrane type1-matrix metalloproteinase (MT1-MMP) using superparamagnetic nanoparticles in human liver cancer cells

Abstract

Keywords

UN SDGs

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