Abstract
Background: The efficiency of lipid extraction by different kinds of solvents from stratum corneum may vary. Objective: To assess the efficiency of lipid extraction by several solvents from the stratum corneum, the total lipids weights were measured by an electronic microbalance, and lipids compositions of a stratum corneum were analyzed by thin layer chromatography (TLC) after pretreatment of several solvents, respectively. Methods: Stratum corneums separated from circumcised prepuce were pretreated with acetone, petroleum ether, or distilled water for 10 minutes. Lipids of stratum corneum were extracted with methanol-chloroform-H2O mixture (4:2:1.6, v/v, Bligh-Dyer solvent). Lipids weights were weighed, and the ratio of lipid weight and wet weight of stratum corneum were measured. TLC was performed and lipids compositions were quantitated by photodensitometer. Lipids extraction in vivo was performed on both forearms. After two times' stripping with sellotape, lipids were extracted with the solvents using cup method. These were dried and lipids weights were weighed. Results: The efficiency of lipid extraction from the stratum corneum of circumcised prepuce were acetone, petroleum ether, and distilled water in decreasing order. All groups were similar in the compositions of the stratum corneum lipids except for those of cholesterol esters and sphingolipids. The efficiency of lipid extraction from in vivo skin were ranked in order from petroleum ether, acetone, down to distilled water. The efficiency of lipid extraction of petroleum ether and acetone were higher than that of distilled water. Conclusion: The efficiency of lipid extraction is influenced by the kinds of solvents as well as the materials and the methods of extraction.
| Original language | English |
|---|---|
| Pages (from-to) | 830-840 |
| Number of pages | 11 |
| Journal | Korean Journal of Dermatology |
| Volume | 33 |
| Issue number | 5 |
| State | Published - 1995 |
Keywords
- lipid extraction
- solvents
- stratum corneum