The detection of Salmonella typhimurium on shell eggs using a phage-based biosensor

Yating Chai, Suiqiong Li, Shin Horikawa, Wen Shen, Mi Kyung Park, Vitaly J. Vodyanoy, Bryan A. Chin

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

3 Scopus citations

Abstract

This paper presents the direct detection of Salmonella typhimurium on shell eggs using a phage-based magnetoelastic (ME) biosensor. The ME biosensor consists of a ME resonator as the sensor platform and E2 phage as the biorecognition element that is genetically engineered to specifically bind with Salmonella typhimurium. The ME biosensor, which is a wireless sensor, vibrates with a characteristic resonant frequency under an externally applied magnetic field. Multiple sensors can easily be remotely monitored. Multiple measurement and control sensors were placed on the shell eggs contaminated by Salmonella typhimurium solutions with different known concentrations. The resonant frequency of sensors before and after the exposure to the spiked shell eggs was measured. The frequency shift of the measurement sensors was significantly different than the control sensors indicating Salmonella contamination. Scanning electron microscopy was used to confirm binding of Salmonella to the sensor surface and the resulting frequency shift results.

Original languageEnglish
Title of host publicationSensing for Agriculture and Food Quality and Safety III
DOIs
StatePublished - 2011
EventSensing for Agriculture and Food Quality and Safety III - Orlando, FL, United States
Duration: 26 Apr 201127 Apr 2011

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume8027
ISSN (Print)0277-786X

Conference

ConferenceSensing for Agriculture and Food Quality and Safety III
Country/TerritoryUnited States
CityOrlando, FL
Period26/04/1127/04/11

Keywords

  • biosensor
  • E2 phage
  • Salmonella typhimurium
  • shell eggs

Fingerprint

Dive into the research topics of 'The detection of Salmonella typhimurium on shell eggs using a phage-based biosensor'. Together they form a unique fingerprint.

Cite this