The effects of red ginseng saponin fraction-A (RGSF-A) on phagocytosis and intracellular signaling in Brucella abortus infected RAW 264.7 cells

Lauren Togonon Arayan; Hannah Leah Simborio; Alisha Wehdnesday Bernardo Reyes; Huynh Tan Hop; Won Gi Min; Hu Jang Lee; Man Hee Rhee; Hong Hee Chang; Suk Kim

doi:10.1093/femsle/fnv070

The effects of red ginseng saponin fraction-A (RGSF-A) on phagocytosis and intracellular signaling in Brucella abortus infected RAW 264.7 cells

Lauren Togonon Arayan, Hannah Leah Simborio, Alisha Wehdnesday Bernardo Reyes, Huynh Tan Hop, Won Gi Min, Hu Jang Lee, Man Hee Rhee, Hong Hee Chang, Suk Kim

Department of Veterinary Medicine

Gyeongsang National University

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

This study indicated that RGSF-A caused a marked reduction in the adherence, internalization and intracellular growth of Brucella abortus in RGSF-A-treated cells. Furthermore, a decline in the intensity of F-actin fluorescence was observed in RGSF-A-treated cells compared with untreated B. abortus-infected cells. In addition, an evaluation of phagocytic signaling proteins by Western blot analysis revealed an apparent reduction of ERK and p38a phosphorylation levels in B. abortus-infected RGSF-A-treated cells compared with the control. Upon intracellular trafficking of the pathogen, a higher number of B. abortus-containing phagosomes colocalized with LAMP-1 in RGSF-A-treated cells compared with control cells.These results strongly suggest that inhibition of B. abortus uptake could be mediated by suppression in the activation of MAPKs signaling proteins phospho-ERK 1/2, and p38 levels. On the other hand, inhibition of intracellular replication results from the enhancement of phagolysosome fusion in host macrophages. This study highlights the phagocytic and intracellular modulating effect of RGSF-A and its potential as an alternative remedy to control B. abortus infection.

Original language	English
Article number	fnv070
Journal	FEMS Microbiology Letters
Volume	362
Issue number	11
DOIs	https://doi.org/10.1093/femsle/fnv070
State	Published - 1 Jun 2015

Keywords

Brucella abortus
Macrophage
Phagocytosis
Red ginseng saponin fraction A

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10.1093/femsle/fnv070

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title = "The effects of red ginseng saponin fraction-A (RGSF-A) on phagocytosis and intracellular signaling in Brucella abortus infected RAW 264.7 cells",

abstract = "This study indicated that RGSF-A caused a marked reduction in the adherence, internalization and intracellular growth of Brucella abortus in RGSF-A-treated cells. Furthermore, a decline in the intensity of F-actin fluorescence was observed in RGSF-A-treated cells compared with untreated B. abortus-infected cells. In addition, an evaluation of phagocytic signaling proteins by Western blot analysis revealed an apparent reduction of ERK and p38a phosphorylation levels in B. abortus-infected RGSF-A-treated cells compared with the control. Upon intracellular trafficking of the pathogen, a higher number of B. abortus-containing phagosomes colocalized with LAMP-1 in RGSF-A-treated cells compared with control cells.These results strongly suggest that inhibition of B. abortus uptake could be mediated by suppression in the activation of MAPKs signaling proteins phospho-ERK 1/2, and p38 levels. On the other hand, inhibition of intracellular replication results from the enhancement of phagolysosome fusion in host macrophages. This study highlights the phagocytic and intracellular modulating effect of RGSF-A and its potential as an alternative remedy to control B. abortus infection.",

keywords = "Brucella abortus, Macrophage, Phagocytosis, Red ginseng saponin fraction A",

author = "Arayan, {Lauren Togonon} and Simborio, {Hannah Leah} and Reyes, {Alisha Wehdnesday Bernardo} and Hop, {Huynh Tan} and Min, {Won Gi} and Lee, {Hu Jang} and Rhee, {Man Hee} and Chang, {Hong Hee} and Suk Kim",

note = "Publisher Copyright: {\textcopyright} FEMS 2015.",

year = "2015",

month = jun,

day = "1",

doi = "10.1093/femsle/fnv070",

language = "English",

volume = "362",

journal = "FEMS Microbiology Letters",

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The effects of red ginseng saponin fraction-A (RGSF-A) on phagocytosis and intracellular signaling in Brucella abortus infected RAW 264.7 cells. / Arayan, Lauren Togonon; Simborio, Hannah Leah; Reyes, Alisha Wehdnesday Bernardo et al.
In: FEMS Microbiology Letters, Vol. 362, No. 11, fnv070, 01.06.2015.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - The effects of red ginseng saponin fraction-A (RGSF-A) on phagocytosis and intracellular signaling in Brucella abortus infected RAW 264.7 cells

AU - Arayan, Lauren Togonon

AU - Simborio, Hannah Leah

AU - Reyes, Alisha Wehdnesday Bernardo

AU - Hop, Huynh Tan

AU - Min, Won Gi

AU - Lee, Hu Jang

AU - Rhee, Man Hee

AU - Chang, Hong Hee

AU - Kim, Suk

PY - 2015/6/1

Y1 - 2015/6/1

N2 - This study indicated that RGSF-A caused a marked reduction in the adherence, internalization and intracellular growth of Brucella abortus in RGSF-A-treated cells. Furthermore, a decline in the intensity of F-actin fluorescence was observed in RGSF-A-treated cells compared with untreated B. abortus-infected cells. In addition, an evaluation of phagocytic signaling proteins by Western blot analysis revealed an apparent reduction of ERK and p38a phosphorylation levels in B. abortus-infected RGSF-A-treated cells compared with the control. Upon intracellular trafficking of the pathogen, a higher number of B. abortus-containing phagosomes colocalized with LAMP-1 in RGSF-A-treated cells compared with control cells.These results strongly suggest that inhibition of B. abortus uptake could be mediated by suppression in the activation of MAPKs signaling proteins phospho-ERK 1/2, and p38 levels. On the other hand, inhibition of intracellular replication results from the enhancement of phagolysosome fusion in host macrophages. This study highlights the phagocytic and intracellular modulating effect of RGSF-A and its potential as an alternative remedy to control B. abortus infection.

AB - This study indicated that RGSF-A caused a marked reduction in the adherence, internalization and intracellular growth of Brucella abortus in RGSF-A-treated cells. Furthermore, a decline in the intensity of F-actin fluorescence was observed in RGSF-A-treated cells compared with untreated B. abortus-infected cells. In addition, an evaluation of phagocytic signaling proteins by Western blot analysis revealed an apparent reduction of ERK and p38a phosphorylation levels in B. abortus-infected RGSF-A-treated cells compared with the control. Upon intracellular trafficking of the pathogen, a higher number of B. abortus-containing phagosomes colocalized with LAMP-1 in RGSF-A-treated cells compared with control cells.These results strongly suggest that inhibition of B. abortus uptake could be mediated by suppression in the activation of MAPKs signaling proteins phospho-ERK 1/2, and p38 levels. On the other hand, inhibition of intracellular replication results from the enhancement of phagolysosome fusion in host macrophages. This study highlights the phagocytic and intracellular modulating effect of RGSF-A and its potential as an alternative remedy to control B. abortus infection.

KW - Brucella abortus

KW - Macrophage

KW - Phagocytosis

KW - Red ginseng saponin fraction A

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DO - 10.1093/femsle/fnv070

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AN - SCOPUS:84948977043

SN - 0378-1097

VL - 362

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

IS - 11

M1 - fnv070

ER -

The effects of red ginseng saponin fraction-A (RGSF-A) on phagocytosis and intracellular signaling in Brucella abortus infected RAW 264.7 cells

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