TY - JOUR
T1 - The expression patterns of RGS transcripts in platelets
AU - Kim, Sung Dae
AU - Sung, Hye Jin
AU - Park, Sun Kyu
AU - Kim, Tae Wan
AU - Park, Seung Chun
AU - Kim, Sang Keun
AU - Cho, Jae Youl
AU - Rhee, Man Hee
PY - 2006/11/1
Y1 - 2006/11/1
N2 - Regulators of G protein signalling (RGS) are involved in the negative regulation of cell activation processes and are involved in the pathophysiology of cardiovascular diseases. To get some further evidence for a role of RGS proteins in platelets, we determined the expression profile of RGS-specific mRNA in rat platelets using reverse transcription-polymerase chain reaction (RT-PCR) with a poly dT18 primer and transcript-specific primers. We found that RGS2, RGS3, RGS5, RGS6, RGS10, RGS14, RGS16 and RGS18, Leukemia-associated Rho-GEF factor (LARG), and Gα interacting protein (GAIP) were differentially expressed in platelets. The highest expression rate was found for RGS18 (about 1.3 fold when compared to GAPDH), followed by LARG, RGS6, RGS10 and RGS16 (0.7 to 0.95), whereas expression rates for RGS2, RGS3, RGS5, RGS14, and GAIP were in a range of 0.1 to 0.3. Our results suggest that G-protein-coupled receptor-mediated signalling in platelet may be regulated mainly by RGS 18, 16, 10, 6, and LARG.
AB - Regulators of G protein signalling (RGS) are involved in the negative regulation of cell activation processes and are involved in the pathophysiology of cardiovascular diseases. To get some further evidence for a role of RGS proteins in platelets, we determined the expression profile of RGS-specific mRNA in rat platelets using reverse transcription-polymerase chain reaction (RT-PCR) with a poly dT18 primer and transcript-specific primers. We found that RGS2, RGS3, RGS5, RGS6, RGS10, RGS14, RGS16 and RGS18, Leukemia-associated Rho-GEF factor (LARG), and Gα interacting protein (GAIP) were differentially expressed in platelets. The highest expression rate was found for RGS18 (about 1.3 fold when compared to GAPDH), followed by LARG, RGS6, RGS10 and RGS16 (0.7 to 0.95), whereas expression rates for RGS2, RGS3, RGS5, RGS14, and GAIP were in a range of 0.1 to 0.3. Our results suggest that G-protein-coupled receptor-mediated signalling in platelet may be regulated mainly by RGS 18, 16, 10, 6, and LARG.
KW - Gene expression
KW - Platelets
KW - RGS
KW - RT-PCR
UR - http://www.scopus.com/inward/record.url?scp=33750496912&partnerID=8YFLogxK
U2 - 10.1080/09537100600758123
DO - 10.1080/09537100600758123
M3 - Article
C2 - 17074726
AN - SCOPUS:33750496912
SN - 0953-7104
VL - 17
SP - 493
EP - 497
JO - Platelets
JF - Platelets
IS - 7
ER -
