Abstract
In this study, we show a convenient MTT assay for detect the susceptibility of yeast-like form of Trichosporon beigelii against antifungal agents. This assay was developed based on mitocondrial respiration by determining reduction of 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) to formazan. Cells of T. beigelii are seeded into 96-well microtiter plates, and antifungal agents, amphotericin B, magainin and CA-ME hybrid peptide were added with various concentrations. After 24 hr incubation, MTT was added, then incubations were continued for 4 hr. Formazan formation was quantified photometrically after extraction of the formazan with acid sodium dodesyl sulfate (SDS). From this assay, we could obtained MICs of antifungal agents against T. beigelii. The presented method can easily be used as an effective methods to assess the antifungal action of various agents on yeasts with minimal amounts of antifungal agents.
| Original language | English |
|---|---|
| Pages (from-to) | 335-337 |
| Number of pages | 3 |
| Journal | Korean Journal of Applied Microbiology and Biotechnology |
| Volume | 25 |
| Issue number | 3 |
| State | Published - Jun 1997 |
Keywords
- Amphotericin B
- Antifungal activity
- Melittin hydrid peptide