TY - JOUR
T1 - The methanothermobacter thermautotrophicus MCM helicase is active as a hexameric ring
AU - Shin, Jae Ho
AU - Heo, Gun Young
AU - Kelman, Zvi
PY - 2009/1/2
Y1 - 2009/1/2
N2 - The minichromosome maintenance (MCM) complex is thought to function as the replicative helicase in archaea and eukarya. The structure of the single MCM protein homologue from the archaeon Methanothermobacter thermautotrophicus is not yet clear, and hexameric, heptameric, octameric, and dodecameric structures, open rings, and filamentous structures have been reported. Using a combination of biochemical and structural analysis, it is shown here that the M. thermautotrophicus MCM helicase is active as a hexamer.
AB - The minichromosome maintenance (MCM) complex is thought to function as the replicative helicase in archaea and eukarya. The structure of the single MCM protein homologue from the archaeon Methanothermobacter thermautotrophicus is not yet clear, and hexameric, heptameric, octameric, and dodecameric structures, open rings, and filamentous structures have been reported. Using a combination of biochemical and structural analysis, it is shown here that the M. thermautotrophicus MCM helicase is active as a hexamer.
UR - http://www.scopus.com/inward/record.url?scp=58649105140&partnerID=8YFLogxK
U2 - 10.1074/jbc.M806803200
DO - 10.1074/jbc.M806803200
M3 - Article
C2 - 19001412
AN - SCOPUS:58649105140
SN - 0021-9258
VL - 284
SP - 540
EP - 546
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 1
ER -