The neuron restrictive silencer factor can act as an activator for dynamin I gene promoter activity in neuronal cells

Jiyun Yoo; Moon Jin Jeong; Sang Seop Lee; Kyung Im Lee; Byoung Mog Kwon; Dong Sun Kim; Young Mee Park; Mi Young Han

doi:10.1006/bbrc.2001.4857

The neuron restrictive silencer factor can act as an activator for dynamin I gene promoter activity in neuronal cells

Jiyun Yoo, Moon Jin Jeong, Sang Seop Lee, Kyung Im Lee, Byoung Mog Kwon, Dong Sun Kim, Young Mee Park, Mi Young Han

Department of Medicine

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

The neuron restrictive silencer element (NRSE) has been identified in several neuronal genes and confers neuron specificity by silencing transcription in nonneuronal cells. We have previously reported that Sp1 and an NF-κB-like element (NE-1) are required for the promoter activity of mouse dynamin I gene. In the present study, we found that the upstream regulatory region of the dynamin I promoter has an NRSE-like sequence and showed that neuron restrictive silencer factor (NRSF) binds to this element in neuronal cells as well as in nonneuronal cells. We also showed that NRSF activates the promoter activity of dynamin I gene in neuronal cells. From the results in this study, we suggest that NRSE might be involved in the neuron restriction of dynamin I expression, and NRSF could act as an activator for promoter activity of dynamin I gene in neuronal cells.

Original language	English
Pages (from-to)	928-932
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	283
Issue number	4
DOIs	https://doi.org/10.1006/bbrc.2001.4857
State	Published - 2001

Keywords

Dynamin I
NRSE
NRSF
NS20Y cells
Promoter

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title = "The neuron restrictive silencer factor can act as an activator for dynamin I gene promoter activity in neuronal cells",

abstract = "The neuron restrictive silencer element (NRSE) has been identified in several neuronal genes and confers neuron specificity by silencing transcription in nonneuronal cells. We have previously reported that Sp1 and an NF-κB-like element (NE-1) are required for the promoter activity of mouse dynamin I gene. In the present study, we found that the upstream regulatory region of the dynamin I promoter has an NRSE-like sequence and showed that neuron restrictive silencer factor (NRSF) binds to this element in neuronal cells as well as in nonneuronal cells. We also showed that NRSF activates the promoter activity of dynamin I gene in neuronal cells. From the results in this study, we suggest that NRSE might be involved in the neuron restriction of dynamin I expression, and NRSF could act as an activator for promoter activity of dynamin I gene in neuronal cells.",

keywords = "Dynamin I, NRSE, NRSF, NS20Y cells, Promoter",

author = "Jiyun Yoo and Jeong, {Moon Jin} and Lee, {Sang Seop} and Lee, {Kyung Im} and Kwon, {Byoung Mog} and Kim, {Dong Sun} and Park, {Young Mee} and Han, {Mi Young}",

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T1 - The neuron restrictive silencer factor can act as an activator for dynamin I gene promoter activity in neuronal cells

AU - Yoo, Jiyun

AU - Jeong, Moon Jin

AU - Lee, Sang Seop

AU - Lee, Kyung Im

AU - Kwon, Byoung Mog

AU - Kim, Dong Sun

AU - Park, Young Mee

AU - Han, Mi Young

PY - 2001

Y1 - 2001

N2 - The neuron restrictive silencer element (NRSE) has been identified in several neuronal genes and confers neuron specificity by silencing transcription in nonneuronal cells. We have previously reported that Sp1 and an NF-κB-like element (NE-1) are required for the promoter activity of mouse dynamin I gene. In the present study, we found that the upstream regulatory region of the dynamin I promoter has an NRSE-like sequence and showed that neuron restrictive silencer factor (NRSF) binds to this element in neuronal cells as well as in nonneuronal cells. We also showed that NRSF activates the promoter activity of dynamin I gene in neuronal cells. From the results in this study, we suggest that NRSE might be involved in the neuron restriction of dynamin I expression, and NRSF could act as an activator for promoter activity of dynamin I gene in neuronal cells.

AB - The neuron restrictive silencer element (NRSE) has been identified in several neuronal genes and confers neuron specificity by silencing transcription in nonneuronal cells. We have previously reported that Sp1 and an NF-κB-like element (NE-1) are required for the promoter activity of mouse dynamin I gene. In the present study, we found that the upstream regulatory region of the dynamin I promoter has an NRSE-like sequence and showed that neuron restrictive silencer factor (NRSF) binds to this element in neuronal cells as well as in nonneuronal cells. We also showed that NRSF activates the promoter activity of dynamin I gene in neuronal cells. From the results in this study, we suggest that NRSE might be involved in the neuron restriction of dynamin I expression, and NRSF could act as an activator for promoter activity of dynamin I gene in neuronal cells.

KW - Dynamin I

KW - NRSE

KW - NRSF

KW - NS20Y cells

KW - Promoter

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JO - Biochemical and Biophysical Research Communications

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IS - 4

ER -

The neuron restrictive silencer factor can act as an activator for dynamin I gene promoter activity in neuronal cells

Abstract

Keywords

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