The newly isolated endophytic fungus Paraconiothyrium sp. LK1 produces ascotoxin

Abdul Latif Khan; Muhammad Hamayun; Javid Hussain; Sang Mo Kang; In Jung Lee

doi:10.3390/molecules17011103

The newly isolated endophytic fungus Paraconiothyrium sp. LK1 produces ascotoxin

Abdul Latif Khan, Muhammad Hamayun, Javid Hussain, Sang Mo Kang, In Jung Lee

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

We have isolated five endophytic fungi from the roots of Capsicum annuum, Cucumis sativus and Glycine max. The culture filtrates (CF) of these endophytes were screened on dwarf mutant rice (Waito-C) and normal rice (Dongjin-byeo). Endophyte CAC- 1A significantly inhibited the growth of Waito-C and Dongjin-byeo. Endophyte CAC-1A was identified as Paraconiothyrium sp. by sequencing the ITS rDNA region and phylogenetic analysis. The ethyl acetate fraction of Paraconiothyrium sp. suppressed the germination of Lactuca sativa and Echinochloa crus-galli seeds. The ethyl acetate fraction of the endophyte was subjected to bioassay-guided isolation and we obtained the phytotoxic compound ascotoxin (1) which was characterized through NMR and GC/MS techniques. Ascotoxin revealed 100% inhibitory effects on seed germination of Echinochloa crus-galli. Compound (1) was isolated for the first time from Paraconiothyrium sp.

Original language	English
Pages (from-to)	1103-1112
Number of pages	10
Journal	Molecules
Volume	17
Issue number	1
DOIs	https://doi.org/10.3390/molecules17011103
State	Published - Jan 2012

Keywords

Ascotoxin
Endophytic fungi
Growth inhibitory effect
Paraconiothyrium sp.

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10.3390/molecules17011103

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title = "The newly isolated endophytic fungus Paraconiothyrium sp. LK1 produces ascotoxin",

abstract = "We have isolated five endophytic fungi from the roots of Capsicum annuum, Cucumis sativus and Glycine max. The culture filtrates (CF) of these endophytes were screened on dwarf mutant rice (Waito-C) and normal rice (Dongjin-byeo). Endophyte CAC- 1A significantly inhibited the growth of Waito-C and Dongjin-byeo. Endophyte CAC-1A was identified as Paraconiothyrium sp. by sequencing the ITS rDNA region and phylogenetic analysis. The ethyl acetate fraction of Paraconiothyrium sp. suppressed the germination of Lactuca sativa and Echinochloa crus-galli seeds. The ethyl acetate fraction of the endophyte was subjected to bioassay-guided isolation and we obtained the phytotoxic compound ascotoxin (1) which was characterized through NMR and GC/MS techniques. Ascotoxin revealed 100% inhibitory effects on seed germination of Echinochloa crus-galli. Compound (1) was isolated for the first time from Paraconiothyrium sp.",

keywords = "Ascotoxin, Endophytic fungi, Growth inhibitory effect, Paraconiothyrium sp.",

author = "Khan, {Abdul Latif} and Muhammad Hamayun and Javid Hussain and Kang, {Sang Mo} and Lee, {In Jung}",

year = "2012",

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AU - Khan, Abdul Latif

AU - Hamayun, Muhammad

AU - Hussain, Javid

AU - Kang, Sang Mo

AU - Lee, In Jung

PY - 2012/1

Y1 - 2012/1

N2 - We have isolated five endophytic fungi from the roots of Capsicum annuum, Cucumis sativus and Glycine max. The culture filtrates (CF) of these endophytes were screened on dwarf mutant rice (Waito-C) and normal rice (Dongjin-byeo). Endophyte CAC- 1A significantly inhibited the growth of Waito-C and Dongjin-byeo. Endophyte CAC-1A was identified as Paraconiothyrium sp. by sequencing the ITS rDNA region and phylogenetic analysis. The ethyl acetate fraction of Paraconiothyrium sp. suppressed the germination of Lactuca sativa and Echinochloa crus-galli seeds. The ethyl acetate fraction of the endophyte was subjected to bioassay-guided isolation and we obtained the phytotoxic compound ascotoxin (1) which was characterized through NMR and GC/MS techniques. Ascotoxin revealed 100% inhibitory effects on seed germination of Echinochloa crus-galli. Compound (1) was isolated for the first time from Paraconiothyrium sp.

AB - We have isolated five endophytic fungi from the roots of Capsicum annuum, Cucumis sativus and Glycine max. The culture filtrates (CF) of these endophytes were screened on dwarf mutant rice (Waito-C) and normal rice (Dongjin-byeo). Endophyte CAC- 1A significantly inhibited the growth of Waito-C and Dongjin-byeo. Endophyte CAC-1A was identified as Paraconiothyrium sp. by sequencing the ITS rDNA region and phylogenetic analysis. The ethyl acetate fraction of Paraconiothyrium sp. suppressed the germination of Lactuca sativa and Echinochloa crus-galli seeds. The ethyl acetate fraction of the endophyte was subjected to bioassay-guided isolation and we obtained the phytotoxic compound ascotoxin (1) which was characterized through NMR and GC/MS techniques. Ascotoxin revealed 100% inhibitory effects on seed germination of Echinochloa crus-galli. Compound (1) was isolated for the first time from Paraconiothyrium sp.

KW - Ascotoxin

KW - Endophytic fungi

KW - Growth inhibitory effect

KW - Paraconiothyrium sp.

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SP - 1103

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JO - Molecules

JF - Molecules

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ER -

The newly isolated endophytic fungus Paraconiothyrium sp. LK1 produces ascotoxin

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Keywords

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