The parthenogenetic activation of canine oocytes with Ca-EDTA by various culture periods and concentrations

Sang Ryeul Lee, Jae Wook Kim, Bong Soo Kim, Myoung Ok Kim, Sung Hyun Kim, Dong Hun Yoo, Mi Jung Shin, Sanggyu Lee, Young Sik Park, Yong Bok Park, Ji Hong Ha, Zae Young Ryoo

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9 Scopus citations

Abstract

In the present study, canine oocytes were exposed to various concentrations of and durations of exposure to EDTA saturated with Ca2+ (Ca-EDTA), a cell membrane-impermeable metal ion chelator, to determine if parthenogenetic activation could be induced. When oocytes were cultured for 48 or 72 h in parthenogenetic activation medium (PAM) without Ca-EDTA (control) or PAM supplemented with 1 or 5 mM Ca-EDTA, the highest rate of pronuclear formation (PN) was obtained in oocytes cultured in 1 mM Ca-EDTA for 48 h (8.0%; P < 0.05). There was no pronuclear formation in the control group (PAM without Ca-EDTA). Oocytes treated with 5 mM Ca-EDTA for 48 h or 1 mM Ca-EDTA for 72 h formed a parthenogenetic pronucleus (3.1 and 4.5, respectively). However, there was no pronuclear formation in oocytes treated with 5 mM Ca-EDTA for 72 h. In summary, exposure to Ca-EDTA can induce pronuclear formation in canine oocytes.

Original languageEnglish
Pages (from-to)698-703
Number of pages6
JournalTheriogenology
Volume67
Issue number4
DOIs
StatePublished - 1 Mar 2007

Keywords

  • Ca-EDTA
  • Canine oocyte
  • Metaphase II
  • Parthenogenesis
  • Pronucleus

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