Abstract
In the present study, canine oocytes were exposed to various concentrations of and durations of exposure to EDTA saturated with Ca2+ (Ca-EDTA), a cell membrane-impermeable metal ion chelator, to determine if parthenogenetic activation could be induced. When oocytes were cultured for 48 or 72 h in parthenogenetic activation medium (PAM) without Ca-EDTA (control) or PAM supplemented with 1 or 5 mM Ca-EDTA, the highest rate of pronuclear formation (PN) was obtained in oocytes cultured in 1 mM Ca-EDTA for 48 h (8.0%; P < 0.05). There was no pronuclear formation in the control group (PAM without Ca-EDTA). Oocytes treated with 5 mM Ca-EDTA for 48 h or 1 mM Ca-EDTA for 72 h formed a parthenogenetic pronucleus (3.1 and 4.5, respectively). However, there was no pronuclear formation in oocytes treated with 5 mM Ca-EDTA for 72 h. In summary, exposure to Ca-EDTA can induce pronuclear formation in canine oocytes.
Original language | English |
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Pages (from-to) | 698-703 |
Number of pages | 6 |
Journal | Theriogenology |
Volume | 67 |
Issue number | 4 |
DOIs | |
State | Published - 1 Mar 2007 |
Keywords
- Ca-EDTA
- Canine oocyte
- Metaphase II
- Parthenogenesis
- Pronucleus