Tracking of dendritic cell migration into lymph nodes using molecular imaging with sodium iodide symporter and enhanced firefly luciferase genes

Ho Won Lee; Seung Yun Yoon; Thoudam Debraj Singh; Yoon Ju Choi; Hong Je Lee; Ji Young Park; Shin Young Jeong; Sang Woo Lee; Jeoung Hee Ha; Byeong Cheol Ahn; Yong Hyun Jeon; Jaetae Lee

doi:10.1038/srep09865

Tracking of dendritic cell migration into lymph nodes using molecular imaging with sodium iodide symporter and enhanced firefly luciferase genes

Ho Won Lee, Seung Yun Yoon, Thoudam Debraj Singh, Yoon Ju Choi, Hong Je Lee, Ji Young Park, Shin Young Jeong, Sang Woo Lee, Jeoung Hee Ha, Byeong Cheol Ahn, Yong Hyun Jeon, Jaetae Lee

Department of Medicine

Research output: Contribution to journal › Article › peer-review

42 Scopus citations

Abstract

We sought to evaluate the feasibility of molecular imaging using the human sodium iodide symporter (hNIS) gene as a reporter, in addition to the enhanced firefly luciferase (effluc) gene, for tracking dendritic cell (DCs) migration in living mice. A murine dendritic cell line (DC2.4) co-expressing hNIS and effluc genes (DC/NF) was established. For the DC-tracking study, mice received either parental DCs or DC/NF cells in the left or right footpad, respectively, and combined I-124 PET/CT and bioluminescence imaging (BLI) were performed. In vivo PET/CT imaging with I-124 revealed higher activity of the radiotracer in the draining popliteal lymph nodes (DPLN) of the DC/NF injection site at day 1 than DC injection site (p < 0.05). The uptake value further increased at day 4 (p < 0.005). BLI also demonstrated migration of DC/NF cells to the DPLNs at day 1 post-injection, and signals at the DPLNs were much higher at day 4. These data support the feasibility of hNIS reporter gene imaging in the tracking of DC migration to lymphoid organs in living mice. DCs expressing the NIS reporter gene could be a useful tool to optimize various strategies of cell-based immunotherapy.

Original language	English
Article number	9865
Journal	Scientific Reports
Volume	5
DOIs	https://doi.org/10.1038/srep09865
State	Published - 14 May 2015

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Lee, H. W., Yoon, S. Y., Singh, T. D., Choi, Y. J., Lee, H. J., Park, J. Y., Jeong, S. Y., Lee, S. W., Ha, J. H., Ahn, B. C., Jeon, Y. H., & Lee, J. (2015). Tracking of dendritic cell migration into lymph nodes using molecular imaging with sodium iodide symporter and enhanced firefly luciferase genes. Scientific Reports, 5, Article 9865. https://doi.org/10.1038/srep09865

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title = "Tracking of dendritic cell migration into lymph nodes using molecular imaging with sodium iodide symporter and enhanced firefly luciferase genes",

abstract = "We sought to evaluate the feasibility of molecular imaging using the human sodium iodide symporter (hNIS) gene as a reporter, in addition to the enhanced firefly luciferase (effluc) gene, for tracking dendritic cell (DCs) migration in living mice. A murine dendritic cell line (DC2.4) co-expressing hNIS and effluc genes (DC/NF) was established. For the DC-tracking study, mice received either parental DCs or DC/NF cells in the left or right footpad, respectively, and combined I-124 PET/CT and bioluminescence imaging (BLI) were performed. In vivo PET/CT imaging with I-124 revealed higher activity of the radiotracer in the draining popliteal lymph nodes (DPLN) of the DC/NF injection site at day 1 than DC injection site (p < 0.05). The uptake value further increased at day 4 (p < 0.005). BLI also demonstrated migration of DC/NF cells to the DPLNs at day 1 post-injection, and signals at the DPLNs were much higher at day 4. These data support the feasibility of hNIS reporter gene imaging in the tracking of DC migration to lymphoid organs in living mice. DCs expressing the NIS reporter gene could be a useful tool to optimize various strategies of cell-based immunotherapy.",

author = "Lee, {Ho Won} and Yoon, {Seung Yun} and Singh, {Thoudam Debraj} and Choi, {Yoon Ju} and Lee, {Hong Je} and Park, {Ji Young} and Jeong, {Shin Young} and Lee, {Sang Woo} and Ha, {Jeoung Hee} and Ahn, {Byeong Cheol} and Jeon, {Yong Hyun} and Jaetae Lee",

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AU - Lee, Ho Won

AU - Yoon, Seung Yun

AU - Singh, Thoudam Debraj

AU - Choi, Yoon Ju

AU - Lee, Hong Je

AU - Park, Ji Young

AU - Jeong, Shin Young

AU - Lee, Sang Woo

AU - Ha, Jeoung Hee

AU - Ahn, Byeong Cheol

AU - Jeon, Yong Hyun

AU - Lee, Jaetae

PY - 2015/5/14

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N2 - We sought to evaluate the feasibility of molecular imaging using the human sodium iodide symporter (hNIS) gene as a reporter, in addition to the enhanced firefly luciferase (effluc) gene, for tracking dendritic cell (DCs) migration in living mice. A murine dendritic cell line (DC2.4) co-expressing hNIS and effluc genes (DC/NF) was established. For the DC-tracking study, mice received either parental DCs or DC/NF cells in the left or right footpad, respectively, and combined I-124 PET/CT and bioluminescence imaging (BLI) were performed. In vivo PET/CT imaging with I-124 revealed higher activity of the radiotracer in the draining popliteal lymph nodes (DPLN) of the DC/NF injection site at day 1 than DC injection site (p < 0.05). The uptake value further increased at day 4 (p < 0.005). BLI also demonstrated migration of DC/NF cells to the DPLNs at day 1 post-injection, and signals at the DPLNs were much higher at day 4. These data support the feasibility of hNIS reporter gene imaging in the tracking of DC migration to lymphoid organs in living mice. DCs expressing the NIS reporter gene could be a useful tool to optimize various strategies of cell-based immunotherapy.

AB - We sought to evaluate the feasibility of molecular imaging using the human sodium iodide symporter (hNIS) gene as a reporter, in addition to the enhanced firefly luciferase (effluc) gene, for tracking dendritic cell (DCs) migration in living mice. A murine dendritic cell line (DC2.4) co-expressing hNIS and effluc genes (DC/NF) was established. For the DC-tracking study, mice received either parental DCs or DC/NF cells in the left or right footpad, respectively, and combined I-124 PET/CT and bioluminescence imaging (BLI) were performed. In vivo PET/CT imaging with I-124 revealed higher activity of the radiotracer in the draining popliteal lymph nodes (DPLN) of the DC/NF injection site at day 1 than DC injection site (p < 0.05). The uptake value further increased at day 4 (p < 0.005). BLI also demonstrated migration of DC/NF cells to the DPLNs at day 1 post-injection, and signals at the DPLNs were much higher at day 4. These data support the feasibility of hNIS reporter gene imaging in the tracking of DC migration to lymphoid organs in living mice. DCs expressing the NIS reporter gene could be a useful tool to optimize various strategies of cell-based immunotherapy.

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Tracking of dendritic cell migration into lymph nodes using molecular imaging with sodium iodide symporter and enhanced firefly luciferase genes

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