Transcriptional repression of ANGPT1 by histone H3K9 demethylase KDM3B

Arim Han; Yun Cheol Chae; Jin Woo Park; Kee Beom Kim; Ji Young Kim; Sang Beom Seo

doi:10.5483/BMBRep.2015.48.7.188

Transcriptional repression of ANGPT1 by histone H3K9 demethylase KDM3B

Arim Han, Yun Cheol Chae, Jin Woo Park, Kee Beom Kim, Ji Young Kim, Sang Beom Seo

Chung-Ang University

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Here we report that the H3K9 demethylase KDM3B represses transcription of the angiogenesis regulatory gene, ANGPT1. Negative regulation of ANGPT1 by KDM3B is independent of its Jumonji (JmjC) domain-mediated H3K9 demethylase activity. We demonstrate that KDM3B downregulates ANGPT1 via interaction with SMRT, and suggest that the repressor complex is formed at the promoter area of ANGPT1. Using MTT and wound healing assays, depletion of KDM3B was found to increase cell proliferation and cell motility, indicating that KDM3B has a role in angiogenesis.

Original language	English
Pages (from-to)	401-406
Number of pages	6
Journal	BMB Reports
Volume	48
Issue number	7
DOIs	https://doi.org/10.5483/BMBRep.2015.48.7.188
State	Published - 2015

Keywords

ANGPT1
Cell motility
KDM3B
SMRT
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10.5483/BMBRep.2015.48.7.188

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title = "Transcriptional repression of ANGPT1 by histone H3K9 demethylase KDM3B",

abstract = "Here we report that the H3K9 demethylase KDM3B represses transcription of the angiogenesis regulatory gene, ANGPT1. Negative regulation of ANGPT1 by KDM3B is independent of its Jumonji (JmjC) domain-mediated H3K9 demethylase activity. We demonstrate that KDM3B downregulates ANGPT1 via interaction with SMRT, and suggest that the repressor complex is formed at the promoter area of ANGPT1. Using MTT and wound healing assays, depletion of KDM3B was found to increase cell proliferation and cell motility, indicating that KDM3B has a role in angiogenesis.",

keywords = "ANGPT1, Cell motility, KDM3B, SMRT, Transcription",

author = "Arim Han and Chae, {Yun Cheol} and Park, {Jin Woo} and Kim, {Kee Beom} and Kim, {Ji Young} and Seo, {Sang Beom}",

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AU - Han, Arim

AU - Chae, Yun Cheol

AU - Park, Jin Woo

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AU - Kim, Ji Young

AU - Seo, Sang Beom

PY - 2015

Y1 - 2015

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AB - Here we report that the H3K9 demethylase KDM3B represses transcription of the angiogenesis regulatory gene, ANGPT1. Negative regulation of ANGPT1 by KDM3B is independent of its Jumonji (JmjC) domain-mediated H3K9 demethylase activity. We demonstrate that KDM3B downregulates ANGPT1 via interaction with SMRT, and suggest that the repressor complex is formed at the promoter area of ANGPT1. Using MTT and wound healing assays, depletion of KDM3B was found to increase cell proliferation and cell motility, indicating that KDM3B has a role in angiogenesis.

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KW - Cell motility

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Transcriptional repression of ANGPT1 by histone H3K9 demethylase KDM3B

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