Transcriptome analysis and identification of regulators for long-term plasticity in Aplysia kurodai

Yong Seok Lee; Sun Lim Choi; Tae Hyung Kim; Jin A. Lee; Kyu Kim Hyong; Hyoung Kim; Deok Jin Jang; Jennifer J. Lee; Sunghoon Lee; Sik Sin Gwang; Chang Bae Kim; Yutaka Suzuki; Sumio Sugano; Tai Kubo; Leonid L. Moroz; Eric R. Kandel; Jong Bhak; Bong Kiun Kaang

doi:10.1073/pnas.0808893105

Transcriptome analysis and identification of regulators for long-term plasticity in Aplysia kurodai

Yong Seok Lee, Sun Lim Choi, Tae Hyung Kim, Jin A. Lee, Kyu Kim Hyong, Hyoung Kim, Deok Jin Jang, Jennifer J. Lee, Sunghoon Lee, Sik Sin Gwang, Chang Bae Kim, Yutaka Suzuki, Sumio Sugano, Tai Kubo, Leonid L. Moroz, Eric R. Kandel, Jong Bhak, Bong Kiun Kaang

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23 Scopus citations

Abstract

The marine mollusk Aplysia is a useful model organism for studying the cellular bases of behavior and plasticity. However, molecular studies of Aplysia have been limited by the lack of genomic information. Recently, a large scale characterization of neuronal transcripts was performed in A. californica. Here, we report the analysis of a parallel set of neuronal transcripts from a closely related species A. kurodai found in the northwestern Pacific. We collected 4,859 nonredundant sequences from the nervous system tissue of A. kurodai. By performing microarray and real-time PCR analyses, we found that ApC/EBP, matrilin, antistasin, and eIF3e clones were significantly up-regulated and a BAT1 homologous clone was significantly down-regulated by 5-HT treatment. Among these, we further demonstrated that the Ap-eIF3e plays a key role in 5-HT-induced long-term facilitation (LTF) as a positive regulator.

Original language	English
Pages (from-to)	18602-18607
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	105
Issue number	47
DOIs	https://doi.org/10.1073/pnas.0808893105
State	Published - 25 Nov 2008

Keywords

Expressed sequence tag
Long-term facilitation

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1073/pnas.0808893105

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Lee, Y. S., Choi, S. L., Kim, T. H., Lee, J. A., Hyong, K. K., Kim, H., Jang, D. J., Lee, J. J., Lee, S., Gwang, S. S., Kim, C. B., Suzuki, Y., Sugano, S., Kubo, T., Moroz, L. L., Kandel, E. R., Bhak, J., & Kaang, B. K. (2008). Transcriptome analysis and identification of regulators for long-term plasticity in Aplysia kurodai. Proceedings of the National Academy of Sciences of the United States of America, 105(47), 18602-18607. https://doi.org/10.1073/pnas.0808893105

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title = "Transcriptome analysis and identification of regulators for long-term plasticity in Aplysia kurodai",

abstract = "The marine mollusk Aplysia is a useful model organism for studying the cellular bases of behavior and plasticity. However, molecular studies of Aplysia have been limited by the lack of genomic information. Recently, a large scale characterization of neuronal transcripts was performed in A. californica. Here, we report the analysis of a parallel set of neuronal transcripts from a closely related species A. kurodai found in the northwestern Pacific. We collected 4,859 nonredundant sequences from the nervous system tissue of A. kurodai. By performing microarray and real-time PCR analyses, we found that ApC/EBP, matrilin, antistasin, and eIF3e clones were significantly up-regulated and a BAT1 homologous clone was significantly down-regulated by 5-HT treatment. Among these, we further demonstrated that the Ap-eIF3e plays a key role in 5-HT-induced long-term facilitation (LTF) as a positive regulator.",

keywords = "Expressed sequence tag, Long-term facilitation",

author = "Lee, {Yong Seok} and Choi, {Sun Lim} and Kim, {Tae Hyung} and Lee, {Jin A.} and Hyong, {Kyu Kim} and Hyoung Kim and Jang, {Deok Jin} and Lee, {Jennifer J.} and Sunghoon Lee and Gwang, {Sik Sin} and Kim, {Chang Bae} and Yutaka Suzuki and Sumio Sugano and Tai Kubo and Moroz, {Leonid L.} and Kandel, {Eric R.} and Jong Bhak and Kaang, {Bong Kiun}",

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Lee, YS, Choi, SL, Kim, TH, Lee, JA, Hyong, KK, Kim, H, Jang, DJ, Lee, JJ, Lee, S, Gwang, SS, Kim, CB, Suzuki, Y, Sugano, S, Kubo, T, Moroz, LL, Kandel, ER, Bhak, J & Kaang, BK 2008, 'Transcriptome analysis and identification of regulators for long-term plasticity in Aplysia kurodai', Proceedings of the National Academy of Sciences of the United States of America, vol. 105, no. 47, pp. 18602-18607. https://doi.org/10.1073/pnas.0808893105

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AU - Lee, Yong Seok

AU - Choi, Sun Lim

AU - Kim, Tae Hyung

AU - Lee, Jin A.

AU - Hyong, Kyu Kim

AU - Kim, Hyoung

AU - Jang, Deok Jin

AU - Lee, Jennifer J.

AU - Lee, Sunghoon

AU - Gwang, Sik Sin

AU - Kim, Chang Bae

AU - Suzuki, Yutaka

AU - Sugano, Sumio

AU - Kubo, Tai

AU - Moroz, Leonid L.

AU - Kandel, Eric R.

AU - Bhak, Jong

AU - Kaang, Bong Kiun

PY - 2008/11/25

Y1 - 2008/11/25

N2 - The marine mollusk Aplysia is a useful model organism for studying the cellular bases of behavior and plasticity. However, molecular studies of Aplysia have been limited by the lack of genomic information. Recently, a large scale characterization of neuronal transcripts was performed in A. californica. Here, we report the analysis of a parallel set of neuronal transcripts from a closely related species A. kurodai found in the northwestern Pacific. We collected 4,859 nonredundant sequences from the nervous system tissue of A. kurodai. By performing microarray and real-time PCR analyses, we found that ApC/EBP, matrilin, antistasin, and eIF3e clones were significantly up-regulated and a BAT1 homologous clone was significantly down-regulated by 5-HT treatment. Among these, we further demonstrated that the Ap-eIF3e plays a key role in 5-HT-induced long-term facilitation (LTF) as a positive regulator.

AB - The marine mollusk Aplysia is a useful model organism for studying the cellular bases of behavior and plasticity. However, molecular studies of Aplysia have been limited by the lack of genomic information. Recently, a large scale characterization of neuronal transcripts was performed in A. californica. Here, we report the analysis of a parallel set of neuronal transcripts from a closely related species A. kurodai found in the northwestern Pacific. We collected 4,859 nonredundant sequences from the nervous system tissue of A. kurodai. By performing microarray and real-time PCR analyses, we found that ApC/EBP, matrilin, antistasin, and eIF3e clones were significantly up-regulated and a BAT1 homologous clone was significantly down-regulated by 5-HT treatment. Among these, we further demonstrated that the Ap-eIF3e plays a key role in 5-HT-induced long-term facilitation (LTF) as a positive regulator.

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JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 47

ER -

Transcriptome analysis and identification of regulators for long-term plasticity in Aplysia kurodai

Abstract

Keywords

UN SDGs

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