Transcriptome analysis of the zebrafish mind bomb mutant

Mind bomb (Mib) facilitates Notch signaling pathway by promoting the endocytosis of Notch ligand. The zebrafish mib^ta52b mutant has a defect in its ubiquitin ligase activity which is necessary to inhibit the neurogenesis, resulting in a neuronal hyperplasia. Several genes regulated in the mib^ta52b mutant have been well established, however, there were relatively few reports about the transcriptome profile. To identify the genes differentially expressed in the mib^ta52b mutant, genome-wide analysis was performed using serial analysis of gene expression. Three hundred and thirty-five transcripts were identified whose expressions were significantly altered in the mib^ta52b mutant as compared with the wild-type. Interestingly, it was suggested that the mib^ta52b mutation may affect not only neurogenesis but also mesoderm development. These results provide new insights into Notch signaling pathway.