Transduced PEP-1-ribosomal protein S3 (rpS3) ameliorates 12-O-tetradecanoylphorbol-13-acetate-induced inflammation in mice

Eun Hee Ahn; Dae Won Kim; Hye Won Kang; Min Jae Shin; Moo Ho Won; Joon Kim; Dong Joon Kim; Oh Shin Kwon; Tae Cheon Kang; Kyu Hyung Han; Jinseu Park; Won Sik Eum; Soo Young Choi

doi:10.1016/j.tox.2010.08.004

Transduced PEP-1-ribosomal protein S3 (rpS3) ameliorates 12-O-tetradecanoylphorbol-13-acetate-induced inflammation in mice

Eun Hee Ahn, Dae Won Kim, Hye Won Kang, Min Jae Shin, Moo Ho Won, Joon Kim, Dong Joon Kim, Oh Shin Kwon, Tae Cheon Kang, Kyu Hyung Han, Jinseu Park, Won Sik Eum, Soo Young Choi

Research output: Contribution to journal › Article › peer-review

44 Scopus citations

Abstract

This study investigated the preventive effect of ribosomal protein S3 (rpS3) on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced ear edema in mice. A cell permeable expression vector PEP-1-rpS3 was constructed. Topical application of the vector markedly inhibited TPA-induced expression levels of cyclooxygenase-2 (COX-2) and pro-inflammatory cytokines. Application of PEP-1-rpS3 also resulted in a significant reduction in the activation of nuclear factor-kappa B (NF-. kB) and mitogen-activated protein kinase (MAPK) in TPA-treated ears. These results indicate that PEP-1-rpS3 inhibits inflammatory response cytokines and enzymes by blocking NF-. kB and MAPK, prompting the suggestion that PEP-1-rpS3 can be used as a therapeutic agent against skin inflammation.

Original language	English
Pages (from-to)	192-197
Number of pages	6
Journal	Toxicology
Volume	276
Issue number	3
DOIs	https://doi.org/10.1016/j.tox.2010.08.004
State	Published - 29 Oct 2010

Keywords

Inflammation
MAPK
PEP-1-rpS3
TPA

Access to Document

10.1016/j.tox.2010.08.004

Cite this

@article{28fb7620388445beb69014141e841bf1,

title = "Transduced PEP-1-ribosomal protein S3 (rpS3) ameliorates 12-O-tetradecanoylphorbol-13-acetate-induced inflammation in mice",

abstract = "This study investigated the preventive effect of ribosomal protein S3 (rpS3) on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced ear edema in mice. A cell permeable expression vector PEP-1-rpS3 was constructed. Topical application of the vector markedly inhibited TPA-induced expression levels of cyclooxygenase-2 (COX-2) and pro-inflammatory cytokines. Application of PEP-1-rpS3 also resulted in a significant reduction in the activation of nuclear factor-kappa B (NF-. kB) and mitogen-activated protein kinase (MAPK) in TPA-treated ears. These results indicate that PEP-1-rpS3 inhibits inflammatory response cytokines and enzymes by blocking NF-. kB and MAPK, prompting the suggestion that PEP-1-rpS3 can be used as a therapeutic agent against skin inflammation.",

keywords = "Inflammation, MAPK, PEP-1-rpS3, TPA",

author = "Ahn, {Eun Hee} and Kim, {Dae Won} and Kang, {Hye Won} and Shin, {Min Jae} and Won, {Moo Ho} and Joon Kim and Kim, {Dong Joon} and Kwon, {Oh Shin} and Kang, {Tae Cheon} and Han, {Kyu Hyung} and Jinseu Park and Eum, {Won Sik} and Choi, {Soo Young}",

year = "2010",

month = oct,

day = "29",

doi = "10.1016/j.tox.2010.08.004",

language = "English",

volume = "276",

pages = "192--197",

journal = "Toxicology",

issn = "0300-483X",

publisher = "Elsevier Ireland Ltd",

number = "3",

}

TY - JOUR

T1 - Transduced PEP-1-ribosomal protein S3 (rpS3) ameliorates 12-O-tetradecanoylphorbol-13-acetate-induced inflammation in mice

AU - Ahn, Eun Hee

AU - Kim, Dae Won

AU - Kang, Hye Won

AU - Shin, Min Jae

AU - Won, Moo Ho

AU - Kim, Joon

AU - Kim, Dong Joon

AU - Kwon, Oh Shin

AU - Kang, Tae Cheon

AU - Han, Kyu Hyung

AU - Park, Jinseu

AU - Eum, Won Sik

AU - Choi, Soo Young

PY - 2010/10/29

Y1 - 2010/10/29

N2 - This study investigated the preventive effect of ribosomal protein S3 (rpS3) on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced ear edema in mice. A cell permeable expression vector PEP-1-rpS3 was constructed. Topical application of the vector markedly inhibited TPA-induced expression levels of cyclooxygenase-2 (COX-2) and pro-inflammatory cytokines. Application of PEP-1-rpS3 also resulted in a significant reduction in the activation of nuclear factor-kappa B (NF-. kB) and mitogen-activated protein kinase (MAPK) in TPA-treated ears. These results indicate that PEP-1-rpS3 inhibits inflammatory response cytokines and enzymes by blocking NF-. kB and MAPK, prompting the suggestion that PEP-1-rpS3 can be used as a therapeutic agent against skin inflammation.

AB - This study investigated the preventive effect of ribosomal protein S3 (rpS3) on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced ear edema in mice. A cell permeable expression vector PEP-1-rpS3 was constructed. Topical application of the vector markedly inhibited TPA-induced expression levels of cyclooxygenase-2 (COX-2) and pro-inflammatory cytokines. Application of PEP-1-rpS3 also resulted in a significant reduction in the activation of nuclear factor-kappa B (NF-. kB) and mitogen-activated protein kinase (MAPK) in TPA-treated ears. These results indicate that PEP-1-rpS3 inhibits inflammatory response cytokines and enzymes by blocking NF-. kB and MAPK, prompting the suggestion that PEP-1-rpS3 can be used as a therapeutic agent against skin inflammation.

KW - Inflammation

KW - MAPK

KW - PEP-1-rpS3

KW - TPA

UR - http://www.scopus.com/inward/record.url?scp=77957280271&partnerID=8YFLogxK

U2 - 10.1016/j.tox.2010.08.004

DO - 10.1016/j.tox.2010.08.004

M3 - Article

C2 - 20709134

AN - SCOPUS:77957280271

SN - 0300-483X

VL - 276

SP - 192

EP - 197

JO - Toxicology

JF - Toxicology

IS - 3

ER -

Transduced PEP-1-ribosomal protein S3 (rpS3) ameliorates 12-O-tetradecanoylphorbol-13-acetate-induced inflammation in mice

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this