Transduced Tat-annexin protein suppresses inflammation-associated gene expression in lipopolysaccharide (LPS)-stimulated Raw 264.7 cells

Sun Hwa Lee, Dae Won Kim, Su Sun Back, Hyun Sook Hwang, Eun Young Park, Tae Cheon Kang, Oh Shin Kwon, Jong Hoon Park, Sung Woo Cho, Kyu Hyung Han, Jinseu Park, Won Sik Eum, Soo Young Choi

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Annexin-1 (ANX1) is an anti-inflammatory protein as well as an important modulator in inflammation. However, the precise action of ANX1 remains unclear. To elucidate the protective effects of ANX1 on lipopolysaccharide (LPS)-induced murine macrophage Raw 264.7 cells, we constructed a cell-permeable Tat-ANX1 protein. The transduced Tat-ANX1 protein markedly inhibited the expression of cyclooxygenase-2, production of prostaglandin E 2, and generation of pro-inflammatory cytokines in the cells. Furthermore, transduced Tat-ANX1 protein caused a significant reduction in the activation of nuclear factor-kappa B (NF-kB) and mitogen-activated protein kinase (MAPK). The results indicate that Tat-ANX1 inhibits the production of inflammatory response cytokines and enzymes by blocking NF-kB and MAPK. Therefore, Tat-ANX1 protein may be useful as a therapeutic agent against various inflammatory diseases.

Original languageEnglish
Pages (from-to)484-489
Number of pages6
JournalBMB Reports
Volume44
Issue number7
DOIs
StatePublished - Jul 2011

Keywords

  • Cytokine
  • Inflammation
  • MAPK
  • Protein therapy
  • Tat-ANX1

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