Transgenic arabidopsis expressing acdS gene of Pseudomonas veronii- KJ alleviate the adverse effects of salt and water-logging stress

Several bacterial genes have been identified for the successful transformation of model and crop plants to cope with a variety of stressful conditions. Here, we isolated acdS gene from Pseudomonas veronii- KJ and cloned into Escherichia coli (DE3) for the successful expression of recombinant 1-aminocyclopropane-1-carboxylate (ACC) deaminase. Then, Arabidopsis thaliana was transformed to express the bacterial acdS for the production of ACC deaminase and were compared to control plants in their response to water-logging and salt stress conditions. We used strong and constitutive 35S promoter from cauliflower mosaic virus to over-express the acdS gene. The plasmid (PCB302-3) including the acdS gene was transferred into Agrobacterium tumefaciens (GV3101) and the floral dip method was carried out for successful transformation of A. thaliana. We subsequently, measured the growth and development of transformed and control plants. The transgenic A. thaliana expressing acdS gene revealed increased tolerance to water-logging and salt stress conditions and were less prone to the adverse effects compare to wild type plants.