Transgenic rice plants conferring increased tolerance to rice blast and multiple environmental stresses

Hirofumi Uchimiya; Seiichi Fujii; Jirong Huang; Takaomi Fushimi; Masanori Nishioka; Kyung Min Kim; Maki Kawai Yamada; Takamitsu Kurusu; Kazuyuki Kuchitsu; Michito Tagawa

doi:10.1023/A:1019275218830

Transgenic rice plants conferring increased tolerance to rice blast and multiple environmental stresses

Hirofumi Uchimiya, Seiichi Fujii, Jirong Huang, Takaomi Fushimi, Masanori Nishioka, Kyung Min Kim, Maki Kawai Yamada, Takamitsu Kurusu, Kazuyuki Kuchitsu, Michito Tagawa

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20 Scopus citations

Abstract

We isolated a rice gene (denoted YK1), which showed 78 percent amino acid sequence homology to the maize HM1 gene. A chimeric gene consisting of a promoter and first intron of maize ubiquitin gene and the cDNA of YK1 was introduced into rice via Agrobacterium mediated transformation. Transgenic rice plants overexpressing this chimeric gene were resistant to rice blast (Magnaporthe grisea) disease, which is one of the most serious pathogens in rice. Furthermore, the same transgenic plants conferred high tolerance to several abiotic stresses such as NaCl, UV-C, submergence, and hydrogen peroxide.

Original language	English
Pages (from-to)	25-31
Number of pages	7
Journal	Molecular Breeding
Volume	9
Issue number	1
DOIs	https://doi.org/10.1023/A:1019275218830
State	Published - 2002

Keywords

Abiotic stresses
Blast disease tolerance
NADPH dependent HC-toxin reductase
Oryza sativa

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title = "Transgenic rice plants conferring increased tolerance to rice blast and multiple environmental stresses",

abstract = "We isolated a rice gene (denoted YK1), which showed 78 percent amino acid sequence homology to the maize HM1 gene. A chimeric gene consisting of a promoter and first intron of maize ubiquitin gene and the cDNA of YK1 was introduced into rice via Agrobacterium mediated transformation. Transgenic rice plants overexpressing this chimeric gene were resistant to rice blast (Magnaporthe grisea) disease, which is one of the most serious pathogens in rice. Furthermore, the same transgenic plants conferred high tolerance to several abiotic stresses such as NaCl, UV-C, submergence, and hydrogen peroxide.",

keywords = "Abiotic stresses, Blast disease tolerance, NADPH dependent HC-toxin reductase, Oryza sativa",

author = "Hirofumi Uchimiya and Seiichi Fujii and Jirong Huang and Takaomi Fushimi and Masanori Nishioka and Kim, {Kyung Min} and Yamada, {Maki Kawai} and Takamitsu Kurusu and Kazuyuki Kuchitsu and Michito Tagawa",

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doi = "10.1023/A:1019275218830",

language = "English",

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T1 - Transgenic rice plants conferring increased tolerance to rice blast and multiple environmental stresses

AU - Uchimiya, Hirofumi

AU - Fujii, Seiichi

AU - Huang, Jirong

AU - Fushimi, Takaomi

AU - Nishioka, Masanori

AU - Kim, Kyung Min

AU - Yamada, Maki Kawai

AU - Kurusu, Takamitsu

AU - Kuchitsu, Kazuyuki

AU - Tagawa, Michito

PY - 2002

Y1 - 2002

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AB - We isolated a rice gene (denoted YK1), which showed 78 percent amino acid sequence homology to the maize HM1 gene. A chimeric gene consisting of a promoter and first intron of maize ubiquitin gene and the cDNA of YK1 was introduced into rice via Agrobacterium mediated transformation. Transgenic rice plants overexpressing this chimeric gene were resistant to rice blast (Magnaporthe grisea) disease, which is one of the most serious pathogens in rice. Furthermore, the same transgenic plants conferred high tolerance to several abiotic stresses such as NaCl, UV-C, submergence, and hydrogen peroxide.

KW - Abiotic stresses

KW - Blast disease tolerance

KW - NADPH dependent HC-toxin reductase

KW - Oryza sativa

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Transgenic rice plants conferring increased tolerance to rice blast and multiple environmental stresses

Abstract

Keywords

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