Transient expression of phospholipase D1 during heart development in rats

Changjong Moon; Heechul Kim; Seungjoon Kim; Yongduk Lee; Min Kyoung Shin; Do Sik Min; Taekyun Shin

doi:10.1292/jvms.70.411

Transient expression of phospholipase D1 during heart development in rats

Changjong Moon, Heechul Kim, Seungjoon Kim, Yongduk Lee, Min Kyoung Shin, Do Sik Min, Taekyun Shin

Department of Veterinary Medicine

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

The expression of phospholipase D (PLD) isozymes was examined in the hearts of rats at different stages of development. Immunoprecipitation and Western blot analysis revealed weak PLD1 expression in the hearts of day 17 embryos. The level of PLD1 protein increased transiently 0 and 3 days postpartum, and declined gradually beginning 7 days after birth. Immunohistochemistry revealed weak PLD1 immunostaining in some cells at embryonic day 17. In contrast, some vascular endothelial cells and cardiomyocytes were immunostained typically at days 0, 3, and 7 after birth. After postnatal day 21, weak PLD1 expression was immunodetected in some vascular endothelial cells and cardiomyocytes. This suggests that the PLD1 protein in the heart is strongly associated with the early postnatal development of the heart in rats.

Original language	English
Pages (from-to)	411-413
Number of pages	3
Journal	Journal of Veterinary Medical Science
Volume	70
Issue number	4
DOIs	https://doi.org/10.1292/jvms.70.411
State	Published - Apr 2008

Keywords

Development
Heart
Phospholipase D1

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10.1292/jvms.70.411

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title = "Transient expression of phospholipase D1 during heart development in rats",

abstract = "The expression of phospholipase D (PLD) isozymes was examined in the hearts of rats at different stages of development. Immunoprecipitation and Western blot analysis revealed weak PLD1 expression in the hearts of day 17 embryos. The level of PLD1 protein increased transiently 0 and 3 days postpartum, and declined gradually beginning 7 days after birth. Immunohistochemistry revealed weak PLD1 immunostaining in some cells at embryonic day 17. In contrast, some vascular endothelial cells and cardiomyocytes were immunostained typically at days 0, 3, and 7 after birth. After postnatal day 21, weak PLD1 expression was immunodetected in some vascular endothelial cells and cardiomyocytes. This suggests that the PLD1 protein in the heart is strongly associated with the early postnatal development of the heart in rats.",

keywords = "Development, Heart, Phospholipase D1",

author = "Changjong Moon and Heechul Kim and Seungjoon Kim and Yongduk Lee and Shin, {Min Kyoung} and Min, {Do Sik} and Taekyun Shin",

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AU - Moon, Changjong

AU - Kim, Heechul

AU - Kim, Seungjoon

AU - Lee, Yongduk

AU - Shin, Min Kyoung

AU - Min, Do Sik

AU - Shin, Taekyun

PY - 2008/4

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N2 - The expression of phospholipase D (PLD) isozymes was examined in the hearts of rats at different stages of development. Immunoprecipitation and Western blot analysis revealed weak PLD1 expression in the hearts of day 17 embryos. The level of PLD1 protein increased transiently 0 and 3 days postpartum, and declined gradually beginning 7 days after birth. Immunohistochemistry revealed weak PLD1 immunostaining in some cells at embryonic day 17. In contrast, some vascular endothelial cells and cardiomyocytes were immunostained typically at days 0, 3, and 7 after birth. After postnatal day 21, weak PLD1 expression was immunodetected in some vascular endothelial cells and cardiomyocytes. This suggests that the PLD1 protein in the heart is strongly associated with the early postnatal development of the heart in rats.

AB - The expression of phospholipase D (PLD) isozymes was examined in the hearts of rats at different stages of development. Immunoprecipitation and Western blot analysis revealed weak PLD1 expression in the hearts of day 17 embryos. The level of PLD1 protein increased transiently 0 and 3 days postpartum, and declined gradually beginning 7 days after birth. Immunohistochemistry revealed weak PLD1 immunostaining in some cells at embryonic day 17. In contrast, some vascular endothelial cells and cardiomyocytes were immunostained typically at days 0, 3, and 7 after birth. After postnatal day 21, weak PLD1 expression was immunodetected in some vascular endothelial cells and cardiomyocytes. This suggests that the PLD1 protein in the heart is strongly associated with the early postnatal development of the heart in rats.

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KW - Phospholipase D1

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Transient expression of phospholipase D1 during heart development in rats

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