Triad base pairs containing fluorene unit for quencher-free SNP typing

Jin Ho Ryu; Young Jun Seo; Gil Tae Hwang; Jin Yong Lee; Byeang Hyean Kim

doi:10.1016/j.tet.2006.10.091

Triad base pairs containing fluorene unit for quencher-free SNP typing

Jin Ho Ryu, Young Jun Seo, Gil Tae Hwang, Jin Yong Lee, Byeang Hyean Kim

Research output: Contribution to journal › Article › peer-review

49 Scopus citations

Abstract

We designed an A-selective fluorescent DNA probe, U^FL, which bears a 2-ethynylfluorene moiety covalently attached to the base dU, and incorporated it into a central position in the hairpin loop. We describe photophysical studies of triad base pairs containing an U^FL DNA probe and their application to single-nucleotide polymorphism (SNP) typing using novel quencher-free molecular beacons (MBs). The drastic changes in the fluorescence properties that arise upon changing the nature of the complementary base and FBs suggest that these triad base pairs are the key elements for quencher-free SNP typing.

Original language	English
Pages (from-to)	3538-3547
Number of pages	10
Journal	Tetrahedron
Volume	63
Issue number	17
DOIs	https://doi.org/10.1016/j.tet.2006.10.091
State	Published - 23 Apr 2007

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10.1016/j.tet.2006.10.091

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abstract = "We designed an A-selective fluorescent DNA probe, UFL, which bears a 2-ethynylfluorene moiety covalently attached to the base dU, and incorporated it into a central position in the hairpin loop. We describe photophysical studies of triad base pairs containing an UFL DNA probe and their application to single-nucleotide polymorphism (SNP) typing using novel quencher-free molecular beacons (MBs). The drastic changes in the fluorescence properties that arise upon changing the nature of the complementary base and FBs suggest that these triad base pairs are the key elements for quencher-free SNP typing.",

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AU - Seo, Young Jun

AU - Hwang, Gil Tae

AU - Lee, Jin Yong

AU - Kim, Byeang Hyean

PY - 2007/4/23

Y1 - 2007/4/23

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AB - We designed an A-selective fluorescent DNA probe, UFL, which bears a 2-ethynylfluorene moiety covalently attached to the base dU, and incorporated it into a central position in the hairpin loop. We describe photophysical studies of triad base pairs containing an UFL DNA probe and their application to single-nucleotide polymorphism (SNP) typing using novel quencher-free molecular beacons (MBs). The drastic changes in the fluorescence properties that arise upon changing the nature of the complementary base and FBs suggest that these triad base pairs are the key elements for quencher-free SNP typing.

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JO - Tetrahedron

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ER -

Triad base pairs containing fluorene unit for quencher-free SNP typing

Abstract

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