Triterpenoid saponins of pulsatilla koreana root have inhibition effects of tumor necrosis factor-α secretion in lipopolysaccharide-induced RAW264.7 cells

Wei Li; Yan Ding; Ya Nan Sun; Xi Tao Yan; Seo Young Yang; Chun Whan Choi; Ji Yun Cha; Young Mi Lee; Young Ho Kim

doi:10.1248/cpb.c12-01034

Triterpenoid saponins of pulsatilla koreana root have inhibition effects of tumor necrosis factor-α secretion in lipopolysaccharide-induced RAW264.7 cells

Wei Li, Yan Ding, Ya Nan Sun, Xi Tao Yan, Seo Young Yang, Chun Whan Choi, Ji Yun Cha, Young Mi Lee, Young Ho Kim

Department of Biology Education

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

In the present study, a new oleanane-type triterpenoid saponin, pulsatilloside F (1), along with 21 known compounds (2-22), were isolated from the root of Pulsatilla koreana. Their chemical structures were elucidated by mass, ¹H-, ¹³C-NMR, correlation spectroscopy (COSY), heteronuclear multiple quantum coherence (HMQC) and heteronuclear multiple bond connectivity (HMBC) spectroscopy. Anti-inflammatory effects of the compounds were evaluated in terms of inhibitory of tumor necrosis factor a (TNF-α) secretion in the lipopolysaccharide (LPS)-stimulated murine RAW264.7 macrophage cell line. Compounds 19 and 20 exhibited particularly inhibitory effects with respective IC₅₀ values of 0.32 and 0.65 μm. Compounds 1-4, 7 and 10-13 exhibited inhibitory effects with inhibition rates up to 41.55-73.76% at a concentration of 5 μm, respectively.

Original language	English
Pages (from-to)	471-476
Number of pages	6
Journal	Chemical and Pharmaceutical Bulletin
Volume	61
Issue number	4
DOIs	https://doi.org/10.1248/cpb.c12-01034
State	Published - Apr 2013

Keywords

Anti-inflammatory
Murine RAW264.7 macrophage cell
Pulsatilla koreana
Ranuculaceae
Triterpenoid saponin

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10.1248/cpb.c12-01034

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title = "Triterpenoid saponins of pulsatilla koreana root have inhibition effects of tumor necrosis factor-α secretion in lipopolysaccharide-induced RAW264.7 cells",

abstract = "In the present study, a new oleanane-type triterpenoid saponin, pulsatilloside F (1), along with 21 known compounds (2-22), were isolated from the root of Pulsatilla koreana. Their chemical structures were elucidated by mass, 1H-, 13C-NMR, correlation spectroscopy (COSY), heteronuclear multiple quantum coherence (HMQC) and heteronuclear multiple bond connectivity (HMBC) spectroscopy. Anti-inflammatory effects of the compounds were evaluated in terms of inhibitory of tumor necrosis factor a (TNF-α) secretion in the lipopolysaccharide (LPS)-stimulated murine RAW264.7 macrophage cell line. Compounds 19 and 20 exhibited particularly inhibitory effects with respective IC50 values of 0.32 and 0.65 μm. Compounds 1-4, 7 and 10-13 exhibited inhibitory effects with inhibition rates up to 41.55-73.76% at a concentration of 5 μm, respectively.",

keywords = "Anti-inflammatory, Murine RAW264.7 macrophage cell, Pulsatilla koreana, Ranuculaceae, Triterpenoid saponin",

author = "Wei Li and Yan Ding and Sun, {Ya Nan} and Yan, {Xi Tao} and Yang, {Seo Young} and Choi, {Chun Whan} and Cha, {Ji Yun} and Lee, {Young Mi} and Kim, {Young Ho}",

year = "2013",

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doi = "10.1248/cpb.c12-01034",

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AU - Li, Wei

AU - Ding, Yan

AU - Sun, Ya Nan

AU - Yan, Xi Tao

AU - Yang, Seo Young

AU - Choi, Chun Whan

AU - Cha, Ji Yun

AU - Lee, Young Mi

AU - Kim, Young Ho

PY - 2013/4

Y1 - 2013/4

N2 - In the present study, a new oleanane-type triterpenoid saponin, pulsatilloside F (1), along with 21 known compounds (2-22), were isolated from the root of Pulsatilla koreana. Their chemical structures were elucidated by mass, 1H-, 13C-NMR, correlation spectroscopy (COSY), heteronuclear multiple quantum coherence (HMQC) and heteronuclear multiple bond connectivity (HMBC) spectroscopy. Anti-inflammatory effects of the compounds were evaluated in terms of inhibitory of tumor necrosis factor a (TNF-α) secretion in the lipopolysaccharide (LPS)-stimulated murine RAW264.7 macrophage cell line. Compounds 19 and 20 exhibited particularly inhibitory effects with respective IC50 values of 0.32 and 0.65 μm. Compounds 1-4, 7 and 10-13 exhibited inhibitory effects with inhibition rates up to 41.55-73.76% at a concentration of 5 μm, respectively.

AB - In the present study, a new oleanane-type triterpenoid saponin, pulsatilloside F (1), along with 21 known compounds (2-22), were isolated from the root of Pulsatilla koreana. Their chemical structures were elucidated by mass, 1H-, 13C-NMR, correlation spectroscopy (COSY), heteronuclear multiple quantum coherence (HMQC) and heteronuclear multiple bond connectivity (HMBC) spectroscopy. Anti-inflammatory effects of the compounds were evaluated in terms of inhibitory of tumor necrosis factor a (TNF-α) secretion in the lipopolysaccharide (LPS)-stimulated murine RAW264.7 macrophage cell line. Compounds 19 and 20 exhibited particularly inhibitory effects with respective IC50 values of 0.32 and 0.65 μm. Compounds 1-4, 7 and 10-13 exhibited inhibitory effects with inhibition rates up to 41.55-73.76% at a concentration of 5 μm, respectively.

KW - Anti-inflammatory

KW - Murine RAW264.7 macrophage cell

KW - Pulsatilla koreana

KW - Ranuculaceae

KW - Triterpenoid saponin

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SN - 0009-2363

VL - 61

SP - 471

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JO - Chemical and Pharmaceutical Bulletin

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ER -

Triterpenoid saponins of pulsatilla koreana root have inhibition effects of tumor necrosis factor-α secretion in lipopolysaccharide-induced RAW264.7 cells

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Keywords

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