Ultra-Fast Photonic Digital Polymerase Chain Reaction based on N-Heterocyclic Carbene Self-Assembled Monolayer

Kyung Ho Kim, Sung Eun Seo, Jinyeong Kim, Seon Joo Park, Jai Eun An, Chan Jae Shin, Choong Min Ryu, Sung Woon Lee, Ho Chul Nam, Tae Ho Yoon, Jong Cheol Shin, Yu Kyung Kim, Hanseul Oh, Jung Joo Hong, Brian N. Kim, Kyoung G. Lee, Hyun Seok Song, Sang Hun Lee, Oh Seok Kwon

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

A molecular diagnosis of the respiratory syncytial virus (RSV) without bulky and expensive instrumentation is of great importance for the early detection and prevention in a fast-spreading pandemic. However, the current representative diagnostic methods have the limitation of being time-consuming, cost, the processing time for polymerase chain reaction (PCR), and inaccurate for lateral flow assay (LFA), representatively. Herein, an integrated photonic digital PCR (dPCR) is developed with high-velocity photonic scanner for in situ fluorescence detection by introducing the N-heterocyclic carbene self-assembled monolayer-based Au film to prevent the quenching effect. The on-site rapid molecular diagnostic platform shows the driving of 40 cycles in under 8 min and fluorescence scanning in under 7 min, resulting in a total analysis time within 15 min. In particular, the technology clearly demonstrates the classification of SARS-CoV-2 patients and healthy controls (99% in sensitivity, 98.6% in specificity, and 96.4% in accuracy with RdRp gene), comparing with standard RT-qPCR. This platform can be utilized for prompt point-of-care molecular diagnostics in early diagnosis and large-scale prevention of next pandemic spreading for upcoming infectious diseases and for the distinction diagnosis with other RSV.

Original languageEnglish
Article number2303728
JournalAdvanced Functional Materials
Volume33
Issue number37
DOIs
StatePublished - 12 Sep 2023

Keywords

  • PoCT
  • carbene chemistry
  • dPCR
  • portable devices
  • respiratory syncytial virus

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