Unlocking biological mechanisms with integrative functional genomics approaches

Sehee Yun, Minsoo Noh, Jivin Yu, Hyeon Jai Kim, Chi Chung Hui, Hunsang Lee, Joe Eun Son

Research output: Contribution to journalReview articlepeer-review

1 Scopus citations

Abstract

Reverse genetics offers precise functional insights into genes through the targeted manipulation of gene expression followed by phenotypic assessment. While these approaches have proven effective in model organisms such as Saccharomyces cerevisiae, large-scale genetic manipulations in human cells were historically unfeasible due to methodological limitations. However, recent advancements in functional genomics, particularly clustered regularly interspaced short palindromic repeats (CRISPR)-based screening technologies and next-generation sequencing platforms, have enabled pooled screening technologies that allow massively parallel, unbiased assessments of biological phenomena in human cells. This review provides a comprehensive overview of cutting-edge functional genomic screening technologies applicable to human cells, ranging from short hairpin RNA screens to modern CRISPR screens. Additionally, we explore the integration of CRISPR platforms with single-cell approaches to monitor gene expression, chromatin accessibility, epigenetic regulation, and chromatin architecture following genetic perturbations at the omics level. By offering an in-depth understanding of these genomic screening methods, this review aims to provide insights into more targeted and effective strategies for genomic research and personalized medicine.

Original languageEnglish
Article number100092
JournalMolecules and Cells
Volume47
Issue number8
DOIs
StatePublished - Aug 2024

Keywords

  • Clustered regularly interspaced short palindromic repeats screening
  • Functional genomics
  • Genome-wide screening
  • Reverse genetics
  • Single-cell genomics
  • Systems biology

Fingerprint

Dive into the research topics of 'Unlocking biological mechanisms with integrative functional genomics approaches'. Together they form a unique fingerprint.

Cite this