Use of a restriction enzyme-digested PCR product as substrate for helicase assays.

Jae Ho Shin; John N. Reeve; Zvi Kelman

doi:10.1093/nar/gni009

Use of a restriction enzyme-digested PCR product as substrate for helicase assays.

Jae Ho Shin, John N. Reeve, Zvi Kelman

School of Applied Bioscience

University of Maryland, College Park

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

DNA helicases play essential roles in many cellular processes. The currently available techniques to generate substrates for helicase assays are fairly complicated and need some expertise not available in all laboratories. Here, a PCR-based method to generate a substrate for a helicase assay is described, and its application for several archaeal, bacterial and viral enzymes is demonstrated.

Original language	English
Pages (from-to)	e8
Journal	Nucleic Acids Research
Volume	33
Issue number	1
DOIs	https://doi.org/10.1093/nar/gni009
State	Published - 2005

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10.1093/nar/gni009

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abstract = "DNA helicases play essential roles in many cellular processes. The currently available techniques to generate substrates for helicase assays are fairly complicated and need some expertise not available in all laboratories. Here, a PCR-based method to generate a substrate for a helicase assay is described, and its application for several archaeal, bacterial and viral enzymes is demonstrated.",

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AB - DNA helicases play essential roles in many cellular processes. The currently available techniques to generate substrates for helicase assays are fairly complicated and need some expertise not available in all laboratories. Here, a PCR-based method to generate a substrate for a helicase assay is described, and its application for several archaeal, bacterial and viral enzymes is demonstrated.

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Use of a restriction enzyme-digested PCR product as substrate for helicase assays.

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