Use of SSR markers to complement tests of distinctiveness, uniformity, and stability (DUS) of pepper (Capsicum annuum L.) varieties

Yong Sham Kwon; Je Min Lee; Gi Bum Yi; Seung In Yi; Kyung Min Kim; Eun Hee Soh; Kyung Mi Bae; Eun Kyung Park; In Ho Song; Byung Dong Kim

Use of SSR markers to complement tests of distinctiveness, uniformity, and stability (DUS) of pepper (Capsicum annuum L.) varieties

Yong Sham Kwon, Je Min Lee, Gi Bum Yi, Seung In Yi, Kyung Min Kim, Eun Hee Soh, Kyung Mi Bae, Eun Kyung Park, In Ho Song, Byung Dong Kim

Research output: Contribution to journal › Article › peer-review

75 Scopus citations

Abstract

This study was carried out to assess the potential of SSR markers for variety identification by comparing SSR markers and morphological traits in tests of distinctiveness, uniformity, and stability (DUS) of pepper (Capsicum annuum L.) varieties. Twenty-seven SSR markers were polymorphic in 66 pepper varieties, revealing a total of 89 alleles. Average polymorphism information content (PIC) value was 0.529, ranging from 0.03 to 0.877. Cluster analysis of the band patterns separated the varieties into three groups corresponding to varietal types. Morphological trait-based clustering showed some degree of similarity to dendrogram topologies based on the SSR index. However, no significance correlation was found between the SSR and morphological data. SSR markers could be used to complement a DUS test of a candidate variety and to select complimentary varieties by pre-screening existing varieties in the context of protecting new varieties of pepper.

Original language	English
Pages (from-to)	428-435
Number of pages	8
Journal	Molecules and Cells
Volume	19
Issue number	3
State	Published - 2005

Keywords

DUS Test
Molecular Marker
Pepper
Plant Variety Protection
SSR

Cite this

@article{37e0471014574489bc9ee4312a463926,

title = "Use of SSR markers to complement tests of distinctiveness, uniformity, and stability (DUS) of pepper (Capsicum annuum L.) varieties",

abstract = "This study was carried out to assess the potential of SSR markers for variety identification by comparing SSR markers and morphological traits in tests of distinctiveness, uniformity, and stability (DUS) of pepper (Capsicum annuum L.) varieties. Twenty-seven SSR markers were polymorphic in 66 pepper varieties, revealing a total of 89 alleles. Average polymorphism information content (PIC) value was 0.529, ranging from 0.03 to 0.877. Cluster analysis of the band patterns separated the varieties into three groups corresponding to varietal types. Morphological trait-based clustering showed some degree of similarity to dendrogram topologies based on the SSR index. However, no significance correlation was found between the SSR and morphological data. SSR markers could be used to complement a DUS test of a candidate variety and to select complimentary varieties by pre-screening existing varieties in the context of protecting new varieties of pepper.",

keywords = "DUS Test, Molecular Marker, Pepper, Plant Variety Protection, SSR",

author = "Kwon, {Yong Sham} and Lee, {Je Min} and Yi, {Gi Bum} and Yi, {Seung In} and Kim, {Kyung Min} and Soh, {Eun Hee} and Bae, {Kyung Mi} and Park, {Eun Kyung} and Song, {In Ho} and Kim, {Byung Dong}",

year = "2005",

language = "English",

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journal = "Molecules and Cells",

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TY - JOUR

T1 - Use of SSR markers to complement tests of distinctiveness, uniformity, and stability (DUS) of pepper (Capsicum annuum L.) varieties

AU - Kwon, Yong Sham

AU - Lee, Je Min

AU - Yi, Gi Bum

AU - Yi, Seung In

AU - Kim, Kyung Min

AU - Soh, Eun Hee

AU - Bae, Kyung Mi

AU - Park, Eun Kyung

AU - Song, In Ho

AU - Kim, Byung Dong

PY - 2005

Y1 - 2005

N2 - This study was carried out to assess the potential of SSR markers for variety identification by comparing SSR markers and morphological traits in tests of distinctiveness, uniformity, and stability (DUS) of pepper (Capsicum annuum L.) varieties. Twenty-seven SSR markers were polymorphic in 66 pepper varieties, revealing a total of 89 alleles. Average polymorphism information content (PIC) value was 0.529, ranging from 0.03 to 0.877. Cluster analysis of the band patterns separated the varieties into three groups corresponding to varietal types. Morphological trait-based clustering showed some degree of similarity to dendrogram topologies based on the SSR index. However, no significance correlation was found between the SSR and morphological data. SSR markers could be used to complement a DUS test of a candidate variety and to select complimentary varieties by pre-screening existing varieties in the context of protecting new varieties of pepper.

AB - This study was carried out to assess the potential of SSR markers for variety identification by comparing SSR markers and morphological traits in tests of distinctiveness, uniformity, and stability (DUS) of pepper (Capsicum annuum L.) varieties. Twenty-seven SSR markers were polymorphic in 66 pepper varieties, revealing a total of 89 alleles. Average polymorphism information content (PIC) value was 0.529, ranging from 0.03 to 0.877. Cluster analysis of the band patterns separated the varieties into three groups corresponding to varietal types. Morphological trait-based clustering showed some degree of similarity to dendrogram topologies based on the SSR index. However, no significance correlation was found between the SSR and morphological data. SSR markers could be used to complement a DUS test of a candidate variety and to select complimentary varieties by pre-screening existing varieties in the context of protecting new varieties of pepper.

KW - DUS Test

KW - Molecular Marker

KW - Pepper

KW - Plant Variety Protection

KW - SSR

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M3 - Article

C2 - 15995361

AN - SCOPUS:27244431800

SN - 1016-8478

VL - 19

SP - 428

EP - 435

JO - Molecules and Cells

JF - Molecules and Cells

IS - 3

ER -

Use of SSR markers to complement tests of distinctiveness, uniformity, and stability (DUS) of pepper (Capsicum annuum L.) varieties

Abstract

Keywords

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