Usefulness of Microsatellite Markers for Horse Parentage Testing

The 50 Cheju native horses (CNH) were used to investigate a usefulness of 12 microsatellite markers prior to registration. The microsatellite loci were amplified by a multiplex procedure. The tests were performed using an AB1 310 automatic prism genetic analyzer (Perkin-Elmer, USA). The allele frequencies, heterozygosity and exclusion probabilities (PE) were estimated. Alleles observed with high frequency were ASB2-A,N (0,350), AHT4-O (0.294), AHT5-O (0.302), HMS2-G (0.391), HMS3-P (0.225), HMS6-O (0.486), HMS7-L (0.813), HTG4-M (0.526), HTG6-O (0.732), HTG7-N (0.477), HTG10-I (0.183) and VHL20-P (0.583), The number of alleles was ranged from 4 to 13. The probability of exclusion of wrongly named parent (PE) was estimated using 12 microsatellites, and observed levels of heterozygosity in CNH were ranged from 0.1626 to 0.4988 and from 0.3210 to 0.8860 (mean 0.6831), respectively. The PE estimated with microsatellites was larger (PE = 0.9972) than that with 16 systems of blood types (PE = 0.9656). The cumulated PE obtained by both microsatellites and blood types was 0.9999.