Virus-like particle vaccine protects against H3N2 canine influenza virus in dog

Dong Hun Lee; Sang Woo Bae; Jae Keun Park; Jung Hoon Kwon; Seong Su Yuk; Jae Min Song; Sang Moo Kang; Yong Kuk Kwon; Hwi Yool Kim; Chang Seon Song

doi:10.1016/j.vaccine.2013.05.023

Virus-like particle vaccine protects against H3N2 canine influenza virus in dog

Dong Hun Lee, Sang Woo Bae, Jae Keun Park, Jung Hoon Kwon, Seong Su Yuk, Jae Min Song, Sang Moo Kang, Yong Kuk Kwon, Hwi Yool Kim, Chang Seon Song

Department of Veterinary Medicine

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

In the present study, virus-like particles (VLPs) were evaluated as a candidate veterinary vaccine against canine influenza virus (CIV) subtype H3N2. Specific pathogen-free (SPF) beagle dogs received a single injection of a VLP vaccine containing hemagglutinin (HA) and M1 protein of CIV H3N2 (H3 HA VLP). The vaccine was tested at 3 different doses with an adjuvant and 1 dose without an adjuvant. To evaluate the immunogenicity and protective efficacy of the H3 HA VLP vaccine, we performed hemagglutination inhibition tests to determine serological immune responses and conducted challenge studies using SPF beagle dogs. The addition of Montanide ISA 25 adjuvant significantly increased the immunogenicity of the H3 HA VLP vaccine. The experimental infection study showed that a single dose of H3 HA VLP vaccine induced protection against wild-type virus challenge in dogs. These results provide support for continued development of the VLP as an animal vaccine against influenza virus.

Original language	English
Pages (from-to)	3268-3273
Number of pages	6
Journal	Vaccine
Volume	31
Issue number	32
DOIs	https://doi.org/10.1016/j.vaccine.2013.05.023
State	Published - 2013

Keywords

Canine influenza
H3N2
Vaccine
Virus-like particle

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.vaccine.2013.05.023

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title = "Virus-like particle vaccine protects against H3N2 canine influenza virus in dog",

abstract = "In the present study, virus-like particles (VLPs) were evaluated as a candidate veterinary vaccine against canine influenza virus (CIV) subtype H3N2. Specific pathogen-free (SPF) beagle dogs received a single injection of a VLP vaccine containing hemagglutinin (HA) and M1 protein of CIV H3N2 (H3 HA VLP). The vaccine was tested at 3 different doses with an adjuvant and 1 dose without an adjuvant. To evaluate the immunogenicity and protective efficacy of the H3 HA VLP vaccine, we performed hemagglutination inhibition tests to determine serological immune responses and conducted challenge studies using SPF beagle dogs. The addition of Montanide ISA 25 adjuvant significantly increased the immunogenicity of the H3 HA VLP vaccine. The experimental infection study showed that a single dose of H3 HA VLP vaccine induced protection against wild-type virus challenge in dogs. These results provide support for continued development of the VLP as an animal vaccine against influenza virus.",

keywords = "Canine influenza, H3N2, Vaccine, Virus-like particle",

author = "Lee, {Dong Hun} and Bae, {Sang Woo} and Park, {Jae Keun} and Kwon, {Jung Hoon} and Yuk, {Seong Su} and Song, {Jae Min} and Kang, {Sang Moo} and Kwon, {Yong Kuk} and Kim, {Hwi Yool} and Song, {Chang Seon}",

year = "2013",

doi = "10.1016/j.vaccine.2013.05.023",

language = "English",

volume = "31",

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journal = "Vaccine",

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T1 - Virus-like particle vaccine protects against H3N2 canine influenza virus in dog

AU - Lee, Dong Hun

AU - Bae, Sang Woo

AU - Park, Jae Keun

AU - Kwon, Jung Hoon

AU - Yuk, Seong Su

AU - Song, Jae Min

AU - Kang, Sang Moo

AU - Kwon, Yong Kuk

AU - Kim, Hwi Yool

AU - Song, Chang Seon

PY - 2013

Y1 - 2013

N2 - In the present study, virus-like particles (VLPs) were evaluated as a candidate veterinary vaccine against canine influenza virus (CIV) subtype H3N2. Specific pathogen-free (SPF) beagle dogs received a single injection of a VLP vaccine containing hemagglutinin (HA) and M1 protein of CIV H3N2 (H3 HA VLP). The vaccine was tested at 3 different doses with an adjuvant and 1 dose without an adjuvant. To evaluate the immunogenicity and protective efficacy of the H3 HA VLP vaccine, we performed hemagglutination inhibition tests to determine serological immune responses and conducted challenge studies using SPF beagle dogs. The addition of Montanide ISA 25 adjuvant significantly increased the immunogenicity of the H3 HA VLP vaccine. The experimental infection study showed that a single dose of H3 HA VLP vaccine induced protection against wild-type virus challenge in dogs. These results provide support for continued development of the VLP as an animal vaccine against influenza virus.

AB - In the present study, virus-like particles (VLPs) were evaluated as a candidate veterinary vaccine against canine influenza virus (CIV) subtype H3N2. Specific pathogen-free (SPF) beagle dogs received a single injection of a VLP vaccine containing hemagglutinin (HA) and M1 protein of CIV H3N2 (H3 HA VLP). The vaccine was tested at 3 different doses with an adjuvant and 1 dose without an adjuvant. To evaluate the immunogenicity and protective efficacy of the H3 HA VLP vaccine, we performed hemagglutination inhibition tests to determine serological immune responses and conducted challenge studies using SPF beagle dogs. The addition of Montanide ISA 25 adjuvant significantly increased the immunogenicity of the H3 HA VLP vaccine. The experimental infection study showed that a single dose of H3 HA VLP vaccine induced protection against wild-type virus challenge in dogs. These results provide support for continued development of the VLP as an animal vaccine against influenza virus.

KW - Canine influenza

KW - H3N2

KW - Vaccine

KW - Virus-like particle

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DO - 10.1016/j.vaccine.2013.05.023

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AN - SCOPUS:84879461148

SN - 0264-410X

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EP - 3273

JO - Vaccine

JF - Vaccine

IS - 32

ER -

Virus-like particle vaccine protects against H3N2 canine influenza virus in dog

Abstract

Keywords

UN SDGs

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