TY - JOUR
T1 - Voltage-dependent anion channels are a key factor of male fertility
AU - Kwon, Woo Sung
AU - Park, Yoo Jin
AU - Mohamed, El Sayed A.
AU - Pang, Myung Geol
PY - 2013/2
Y1 - 2013/2
N2 - Objective: To examine how voltage-dependent anion channels (VDACs) regulate sperm function in capacitation conditions. Design: Experimental prospective study. Setting: Academic research laboratory. Animal(s): Male ICR and female B6D2F1/CrljOri mice (8-12 weeks old). Intervention(s): Female mice were superovulated with 5 IU of pregnant mare serum gonadotropin given IP and 5 IU of hCG given IP 48 hours later. Oocytes were applied to assess fertilization and embryo development. Main Outcome Measure(s): Immunofluorescence assay, computer-Assisted sperm analysis, hypo-osmotic swelling test, combined Hoechst 33258/chlortetracycline fluorescence assessment of capacitation status, measurement of [Ca2+]i and [pH]i, Western blotting, and IVF. Result(s): VDAC2 was localized on the acrosomal region and principal piece, while VDAC3 was localized on the acrosomal region and midpiece. Blocking VDAC with DIDS (500 μM) significantly decreased motility, viability, acrosome reaction, capacitation, tyrosine phosphorylation, fertilization, and embryo development regardless of Ca2+. However, the most severe decreases were observed in the presence (+) of DIDS and absence (-) of Ca2+, respectively. A significant decrease in [Ca 2+]i concentration was observed in (-) DIDS, while [pH]i was significantly increased in (-) DIDS regardless of Ca 2+. However, a significantly elevated [pH]i was observed in (+) Ca2+. Conclusion(s): Abnormal regulation of VDACs negatively affected sperm function. Thus, VDACs may be key regulators of the fertilization ability of spermatozoa.
AB - Objective: To examine how voltage-dependent anion channels (VDACs) regulate sperm function in capacitation conditions. Design: Experimental prospective study. Setting: Academic research laboratory. Animal(s): Male ICR and female B6D2F1/CrljOri mice (8-12 weeks old). Intervention(s): Female mice were superovulated with 5 IU of pregnant mare serum gonadotropin given IP and 5 IU of hCG given IP 48 hours later. Oocytes were applied to assess fertilization and embryo development. Main Outcome Measure(s): Immunofluorescence assay, computer-Assisted sperm analysis, hypo-osmotic swelling test, combined Hoechst 33258/chlortetracycline fluorescence assessment of capacitation status, measurement of [Ca2+]i and [pH]i, Western blotting, and IVF. Result(s): VDAC2 was localized on the acrosomal region and principal piece, while VDAC3 was localized on the acrosomal region and midpiece. Blocking VDAC with DIDS (500 μM) significantly decreased motility, viability, acrosome reaction, capacitation, tyrosine phosphorylation, fertilization, and embryo development regardless of Ca2+. However, the most severe decreases were observed in the presence (+) of DIDS and absence (-) of Ca2+, respectively. A significant decrease in [Ca 2+]i concentration was observed in (-) DIDS, while [pH]i was significantly increased in (-) DIDS regardless of Ca 2+. However, a significantly elevated [pH]i was observed in (+) Ca2+. Conclusion(s): Abnormal regulation of VDACs negatively affected sperm function. Thus, VDACs may be key regulators of the fertilization ability of spermatozoa.
KW - DIDS
KW - male fertility
KW - sperm function
KW - VDAC
UR - http://www.scopus.com/inward/record.url?scp=84873287245&partnerID=8YFLogxK
U2 - 10.1016/j.fertnstert.2012.09.021
DO - 10.1016/j.fertnstert.2012.09.021
M3 - Article
C2 - 23062735
AN - SCOPUS:84873287245
SN - 0015-0282
VL - 99
SP - 354
EP - 361
JO - Fertility and Sterility
JF - Fertility and Sterility
IS - 2
ER -