TY - JOUR
T1 - Voltammetric Aptamer-Based Biochip Featuring Poly Gallic Acid/SnO2–MoO3 Nanocomposite for Selective Tryptophan Detection
T2 - Potential Application in Alzheimer's Diagnosis
AU - Lee, Seung Hyeon
AU - Nde, Dieudonne Tanue
AU - Li, Jingjing
AU - Koh, Eunchan
AU - Lu, Jiaran
AU - Lee, Myungseob
AU - Karuppiah, Chelladurai
AU - Lee, Hye Jin
N1 - Publisher Copyright:
© The Korean BioChip Society 2024.
PY - 2024/12
Y1 - 2024/12
N2 - Tryptophan (Trp) levels are associated with Alzheimer's disease (AD), suggesting its utility as a diagnostic marker in AD patients. In this study, a disposable screen-printed carbon electrode modified with an aptamer, poly gallic acid, and SnO2–MoO3 nanoparticles (Apt/p-GA/SnO2–MoO3/SPCE) was demonstrated for voltammetric selective detection of Trp in clinical plasma samples. X-ray photoelectron and impedance spectroscopy, along with cyclic voltammetry, revealed that SnO2–MoO3 nanoparticles enlarged an effective surface area, while p-GA enhanced aptamer immobilization leading to selectivity for target molecules. The synergistic effects of these components promote excellent electron transfer to Trp. Differential pulse voltammetric measurements for Trp oxidation with Apt/p-GA/SnO2–MoO3/SPCE provided a wide linear range (0.5–100 μM) and a low limit of detection (0.189 μM) with a reasonable sensitivity (0.352 μA μM−1 cm−2), comparable to the reported single metal oxide-based composite electrodes. Further application of the aptasensor to clinical plasma samples from healthy individuals and AD patients revealed an effective reduction of matrix effects during Trp analysis. The sample analysis results were also closely correlated with those obtained using the enzyme-linked immunosorbent assay, highlighting the accuracy and practical utility of the sensor for clinical assessment.
AB - Tryptophan (Trp) levels are associated with Alzheimer's disease (AD), suggesting its utility as a diagnostic marker in AD patients. In this study, a disposable screen-printed carbon electrode modified with an aptamer, poly gallic acid, and SnO2–MoO3 nanoparticles (Apt/p-GA/SnO2–MoO3/SPCE) was demonstrated for voltammetric selective detection of Trp in clinical plasma samples. X-ray photoelectron and impedance spectroscopy, along with cyclic voltammetry, revealed that SnO2–MoO3 nanoparticles enlarged an effective surface area, while p-GA enhanced aptamer immobilization leading to selectivity for target molecules. The synergistic effects of these components promote excellent electron transfer to Trp. Differential pulse voltammetric measurements for Trp oxidation with Apt/p-GA/SnO2–MoO3/SPCE provided a wide linear range (0.5–100 μM) and a low limit of detection (0.189 μM) with a reasonable sensitivity (0.352 μA μM−1 cm−2), comparable to the reported single metal oxide-based composite electrodes. Further application of the aptasensor to clinical plasma samples from healthy individuals and AD patients revealed an effective reduction of matrix effects during Trp analysis. The sample analysis results were also closely correlated with those obtained using the enzyme-linked immunosorbent assay, highlighting the accuracy and practical utility of the sensor for clinical assessment.
KW - Alzheimer's disease
KW - Binary metal oxides
KW - DNA aptamer
KW - Electrochemical aptasensor
KW - Electropolymerization
KW - Tryptophan
UR - http://www.scopus.com/inward/record.url?scp=85207871728&partnerID=8YFLogxK
U2 - 10.1007/s13206-024-00175-x
DO - 10.1007/s13206-024-00175-x
M3 - Article
AN - SCOPUS:85207871728
SN - 1976-0280
VL - 18
SP - 633
EP - 646
JO - Biochip Journal
JF - Biochip Journal
IS - 4
M1 - 143387
ER -