Wide-field optical coherence microscopy of the mouse brain slice

Eunjung Min; Junwon Lee; Andrey Vavilin; Sunwoo Jung; Sungwon Shin; Jeehyun Kim; Woonggyu Jung

doi:10.1364/OL.40.004420

Wide-field optical coherence microscopy of the mouse brain slice

Eunjung Min, Junwon Lee, Andrey Vavilin, Sunwoo Jung, Sungwon Shin, Jeehyun Kim, Woonggyu Jung

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

The imaging capability of optical coherence microscopy (OCM) has great potential to be used in neuroscience research because it is able to visualize anatomic features of brain tissue without labeling or external contrast agents. However, the field of view of OCM is still narrow, which dilutes the strength of OCM and limits its application. In this study, we present fully automated wide-field OCM for mosaic imaging of sliced mouse brains. A total of 308 segmented OCM images were acquired, stitched, and reconstructed as an en-face brain image after intensive imaging processing. The overall imaging area was 11.2 x 7.0 mm (horizontal x vertical), and the corresponding pixel resolution was 1.2 x 1.2 μm. OCM images were compared to traditional histology stained with Nissl and Luxol fast blue (LFB). In particular, the orientation of the fibers was analyzed and quantified in wide-field OCM.

Original language	English
Pages (from-to)	4420-4423
Number of pages	4
Journal	Optics Letters
Volume	40
Issue number	19
DOIs	https://doi.org/10.1364/OL.40.004420
State	Published - 1 Oct 2015

Access to Document

10.1364/OL.40.004420

Cite this

@article{b889816d15664b13b29e71220a4cc5e1,

title = "Wide-field optical coherence microscopy of the mouse brain slice",

abstract = "The imaging capability of optical coherence microscopy (OCM) has great potential to be used in neuroscience research because it is able to visualize anatomic features of brain tissue without labeling or external contrast agents. However, the field of view of OCM is still narrow, which dilutes the strength of OCM and limits its application. In this study, we present fully automated wide-field OCM for mosaic imaging of sliced mouse brains. A total of 308 segmented OCM images were acquired, stitched, and reconstructed as an en-face brain image after intensive imaging processing. The overall imaging area was 11.2 x 7.0 mm (horizontal x vertical), and the corresponding pixel resolution was 1.2 x 1.2 μm. OCM images were compared to traditional histology stained with Nissl and Luxol fast blue (LFB). In particular, the orientation of the fibers was analyzed and quantified in wide-field OCM.",

author = "Eunjung Min and Junwon Lee and Andrey Vavilin and Sunwoo Jung and Sungwon Shin and Jeehyun Kim and Woonggyu Jung",

note = "Publisher Copyright: {\textcopyright} 2015 Optical Society of America.",

year = "2015",

month = oct,

day = "1",

doi = "10.1364/OL.40.004420",

language = "English",

volume = "40",

pages = "4420--4423",

journal = "Optics Letters",

issn = "0146-9592",

publisher = "Optica Publishing Group (formerly OSA)",

number = "19",

}

TY - JOUR

T1 - Wide-field optical coherence microscopy of the mouse brain slice

AU - Min, Eunjung

AU - Lee, Junwon

AU - Vavilin, Andrey

AU - Jung, Sunwoo

AU - Shin, Sungwon

AU - Kim, Jeehyun

AU - Jung, Woonggyu

PY - 2015/10/1

Y1 - 2015/10/1

N2 - The imaging capability of optical coherence microscopy (OCM) has great potential to be used in neuroscience research because it is able to visualize anatomic features of brain tissue without labeling or external contrast agents. However, the field of view of OCM is still narrow, which dilutes the strength of OCM and limits its application. In this study, we present fully automated wide-field OCM for mosaic imaging of sliced mouse brains. A total of 308 segmented OCM images were acquired, stitched, and reconstructed as an en-face brain image after intensive imaging processing. The overall imaging area was 11.2 x 7.0 mm (horizontal x vertical), and the corresponding pixel resolution was 1.2 x 1.2 μm. OCM images were compared to traditional histology stained with Nissl and Luxol fast blue (LFB). In particular, the orientation of the fibers was analyzed and quantified in wide-field OCM.

AB - The imaging capability of optical coherence microscopy (OCM) has great potential to be used in neuroscience research because it is able to visualize anatomic features of brain tissue without labeling or external contrast agents. However, the field of view of OCM is still narrow, which dilutes the strength of OCM and limits its application. In this study, we present fully automated wide-field OCM for mosaic imaging of sliced mouse brains. A total of 308 segmented OCM images were acquired, stitched, and reconstructed as an en-face brain image after intensive imaging processing. The overall imaging area was 11.2 x 7.0 mm (horizontal x vertical), and the corresponding pixel resolution was 1.2 x 1.2 μm. OCM images were compared to traditional histology stained with Nissl and Luxol fast blue (LFB). In particular, the orientation of the fibers was analyzed and quantified in wide-field OCM.

UR - https://www.scopus.com/pages/publications/84958975368

U2 - 10.1364/OL.40.004420

DO - 10.1364/OL.40.004420

M3 - Article

C2 - 26421546

AN - SCOPUS:84958975368

SN - 0146-9592

VL - 40

SP - 4420

EP - 4423

JO - Optics Letters

JF - Optics Letters

IS - 19

ER -

Wide-field optical coherence microscopy of the mouse brain slice

Abstract

Access to Document

Other files and links

Fingerprint

Cite this