Wisteria floribunda gall extract inhibits cell migration in mouse B16F1 melanoma cells by regulating CD44 expression and GTP-RhoA activity

Jin Chul Heo; Ja Young Park; Jin Man Lee; Kyu Kwon Taeg; Sung Uk Kim; Shin Kyo Chung; Sang Han Lee

doi:10.1016/j.jep.2005.04.036

Wisteria floribunda gall extract inhibits cell migration in mouse B16F1 melanoma cells by regulating CD44 expression and GTP-RhoA activity

Jin Chul Heo, Ja Young Park, Jin Man Lee, Kyu Kwon Taeg, Sung Uk Kim, Shin Kyo Chung, Sang Han Lee

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Extracts from galls grown on Wisteria floribunda are used as an anti-tumoral preparation in oriental traditional medicine. Here, we investigated the molecular mechanism of this anti-tumoral effect by first examining whether the extract inhibited cell migration in a B16 cell-based wound healing assay. The gall extract delayed wound healing in a dose- and time-dependent manner, indicating that one or more components of the fraction inhibited cell migration. Examination of two molecules known to be involved in metastasis, CD44, and RhoA-GTP, revealed that the gall extract decreased CD44 expression in a concentration-dependent manner, and also increased RhoA-GTP activity in comparison to untreated controls. Taken together, these results suggest that the Wisteria gall extract may inhibit cancer cell migration via inhibition of CD44 mRNA expression and activation of the GTP-RhoA protein.

Original language	English
Pages (from-to)	10-14
Number of pages	5
Journal	Journal of Ethnopharmacology
Volume	102
Issue number	1
DOIs	https://doi.org/10.1016/j.jep.2005.04.036
State	Published - 31 Oct 2005

Keywords

CD44
Cell migration
Fabaceae
Melanoma cells
RhoA
Wisteria floribunda

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10.1016/j.jep.2005.04.036

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title = "Wisteria floribunda gall extract inhibits cell migration in mouse B16F1 melanoma cells by regulating CD44 expression and GTP-RhoA activity",

abstract = "Extracts from galls grown on Wisteria floribunda are used as an anti-tumoral preparation in oriental traditional medicine. Here, we investigated the molecular mechanism of this anti-tumoral effect by first examining whether the extract inhibited cell migration in a B16 cell-based wound healing assay. The gall extract delayed wound healing in a dose- and time-dependent manner, indicating that one or more components of the fraction inhibited cell migration. Examination of two molecules known to be involved in metastasis, CD44, and RhoA-GTP, revealed that the gall extract decreased CD44 expression in a concentration-dependent manner, and also increased RhoA-GTP activity in comparison to untreated controls. Taken together, these results suggest that the Wisteria gall extract may inhibit cancer cell migration via inhibition of CD44 mRNA expression and activation of the GTP-RhoA protein.",

keywords = "CD44, Cell migration, Fabaceae, Melanoma cells, RhoA, Wisteria floribunda",

author = "Heo, {Jin Chul} and Park, {Ja Young} and Lee, {Jin Man} and Taeg, {Kyu Kwon} and Kim, {Sung Uk} and Chung, {Shin Kyo} and Lee, {Sang Han}",

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doi = "10.1016/j.jep.2005.04.036",

language = "English",

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T1 - Wisteria floribunda gall extract inhibits cell migration in mouse B16F1 melanoma cells by regulating CD44 expression and GTP-RhoA activity

AU - Heo, Jin Chul

AU - Park, Ja Young

AU - Lee, Jin Man

AU - Taeg, Kyu Kwon

AU - Kim, Sung Uk

AU - Chung, Shin Kyo

AU - Lee, Sang Han

PY - 2005/10/31

Y1 - 2005/10/31

N2 - Extracts from galls grown on Wisteria floribunda are used as an anti-tumoral preparation in oriental traditional medicine. Here, we investigated the molecular mechanism of this anti-tumoral effect by first examining whether the extract inhibited cell migration in a B16 cell-based wound healing assay. The gall extract delayed wound healing in a dose- and time-dependent manner, indicating that one or more components of the fraction inhibited cell migration. Examination of two molecules known to be involved in metastasis, CD44, and RhoA-GTP, revealed that the gall extract decreased CD44 expression in a concentration-dependent manner, and also increased RhoA-GTP activity in comparison to untreated controls. Taken together, these results suggest that the Wisteria gall extract may inhibit cancer cell migration via inhibition of CD44 mRNA expression and activation of the GTP-RhoA protein.

AB - Extracts from galls grown on Wisteria floribunda are used as an anti-tumoral preparation in oriental traditional medicine. Here, we investigated the molecular mechanism of this anti-tumoral effect by first examining whether the extract inhibited cell migration in a B16 cell-based wound healing assay. The gall extract delayed wound healing in a dose- and time-dependent manner, indicating that one or more components of the fraction inhibited cell migration. Examination of two molecules known to be involved in metastasis, CD44, and RhoA-GTP, revealed that the gall extract decreased CD44 expression in a concentration-dependent manner, and also increased RhoA-GTP activity in comparison to untreated controls. Taken together, these results suggest that the Wisteria gall extract may inhibit cancer cell migration via inhibition of CD44 mRNA expression and activation of the GTP-RhoA protein.

KW - CD44

KW - Cell migration

KW - Fabaceae

KW - Melanoma cells

KW - RhoA

KW - Wisteria floribunda

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U2 - 10.1016/j.jep.2005.04.036

DO - 10.1016/j.jep.2005.04.036

M3 - Article

C2 - 16023319

AN - SCOPUS:25444437967

SN - 0378-8741

VL - 102

SP - 10

EP - 14

JO - Journal of Ethnopharmacology

JF - Journal of Ethnopharmacology

IS - 1

ER -

Wisteria floribunda gall extract inhibits cell migration in mouse B16F1 melanoma cells by regulating CD44 expression and GTP-RhoA activity

Abstract

Keywords

UN SDGs

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