Xenoestrogenic chemicals effectively alter sperm functional behavior in mice

Yoo Jin Park, El Sayed A. Mohamed, Woo Sung Kwon, Young Ah You, Buom Yong Ryu, Myung Geol Pang

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Xenoestrogenic compounds (XCs) can disrupt endogenous hormone function and affect sperm function by binding to receptors on sperm membrane. Albeit spermatozoa are potentially a useful model for screening estrogenic activities of endocrine disruptors, high-quality in vitro test system that examination of the XCs effects on sperm function is required. The objective of this study was to compare the effects of XCs (genistein and 4-tert-octylphenol) to those of steroids (estrogen and progesterone) and heparin on in vitro capacitation and acrosome reaction (AR) in mouse spermatozoa. Mouse spermatozoa were incubated with various concentrations (0.001-100 μM) of each chemical for 15 or 30. min, and then capacitation and AR were assessed using chlortetracycline. All chemicals studied effectively alter capacitation and/or AR in mouse spermatozoa with different manner. Therefore, we believed that our system will provide a good in vitro model system to characterize the physiological effect of XCs especially when compared with steroids.

Original languageEnglish
Pages (from-to)418-424
Number of pages7
JournalReproductive Toxicology
Volume32
Issue number4
DOIs
StatePublished - Dec 2011

Keywords

  • Acrosome reaction
  • Capacitation
  • Eendocrine disruptors
  • Estrogen
  • Heparin
  • Progesterone
  • Sperm function

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